Trichoderma viride가 生成하는 纖維素 分解酵素의 分別 및 酵素學的 性質에 關한 硏究 = Studies on Fractionation and Enzymological Properties of Celluloytic Enzymes Produced by Trichoderma viridee
저자
成洛癸 (농화학과)
발행기관
학술지명
진주농과대학 연구논문집(The Research Bulletin of Chinju Agricultural College)
권호사항
발행연도
1971
작성언어
Korean
KDC
520.000
자료형태
학술저널
수록면
1-29(29쪽)
제공처
소장기관
One strain of mold producing powerful cellulolytic enzyme complexes was screened from various strains of molds which were isolated from nature, and its microbiological properties and cellulolytic enzymes were studied. The results obtained were summarized as follows:
1) One strain of mold isolated from Locust acasia (Lobinia pseudacasia linne) showed remarkably high cellulolytic activity on the various substrates. This strain, by its microbiological properties, was identified as Trichoderma viride.
2) It was found that there were five different kinds of cellulolytic enzymes in the cellulase derived from Trzchoderma viride. Their physical and enzymological properties were as follows:
i) The optimum pH of each enzyme was 5.0 and the range of stability with respect to pH was generally from 4.0 to 6.0.
ii) The optimum temperature of CMCase, β-glucosidase and filter paper saccharifying enzyme was 60℃, but that of filter paper disintegrating enzyme was 50℃.
The heat stabilities of β-glucosidase, filter paper disintegrating enzyme, CMCase and filter paper saccharifying enzyme were 30, 40, 50, and 55℃, respectively.
iii) When the concentration of metal ions was 2×10³M. ,the activities of CMCase and filter paper disintegrating enzyme were found to be inhibited by ?? and ??. At the same concentration, filter paper saccharifying enzyme was inhibited by ??, EDTA, ??, and ??, while β-glucosidase was inhitibited by all the above metal ions.
3) The purification and fractionation of the cellulolytic enzyme complexes by organic solvents, and column chromatography with ion exchange resin, were as follows:
i) In the purification, β-glucosidase could be collected completely using a 60% solution of acetone and ethanol. In the case of filter paper disintegrating enzyme, the best result was obtained by the use of an 80% acetone solution.
ii) A 100% yield of these enzymes was obtained by the use of saturated aqueous ammonium sulfate solution(60%).
iii) The cellulolytic enzyme complexes could be separated into a few fractions by chromatography with cellulose powder. Most of the filter paper disintegrating enzyme was absorbed, but the others were eluted.
iv) The cellulolytic enzyme complexes could be separated into filter paper disintegrating enzyme and filter paper saccharifying enzyme by gauze column chromatography.
v) The doted fraction could be separated into CMCase, β-glucosidase and filter paper saccharifying enzyme by column chromatography with DEAE-sephadex A-50, Amberite XE-64 and Sephadex G-200.
vi) The filter paper disintegrating enzyme present in the absorbed fraction was resolved by column chromatography with DEAE-sephadcx A-50.
4) The characteristics of the separated fractions were as follows:
i) The ?? value of CMCase was 0.125% and that of β-glucosidase was 0.045%.
ii) The filter paper saccharifying enzyme and filter paper disintegrating enzyme differed with respect to the distance moved during electrophoresis, and in ?? values obtained in paper chromatography.
iii) The filter paper disintegrating enzyme was completely inactivated by heating at 70℃ for 10 minutes, and filter paper saccharifying enzyme activity reduced by about 80 percent.
iv) The decomposing pattern of filter paper saccharifying enzyme was mainly an exo-type and that of filter paper disintegrating enzyme was an endo-type.
5) It has been previously reported that filter paper disintegrating enzyme is indentical with filter paper saccharifying enzyme: however, the present study indicates that these two enzymes differ in physical and enzymological properties.
Therefore it is suggested that there are five cellulolytic components in Trichoderma viride cellulase.
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