골쇄보가 Streptomycin으로 손상된 생쥐의 배양 섬유모세포에 미치는 영향 = Effect of Drynariae Rhizoma on Cultured Mouse Fibroblast Injured by Streptomycin골쇄보가 Streptomycin으로 손상된 생쥐의 배양 섬유모세포에 미치는 영향

다국어 초록 (Multilingual Abstract)

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다국어 초록 (Multilingual Abstract)

Objectives : To examine the cytotoxicity of streptomycin(STR) on cultured mouse fibroblasts, cytotoxocity-induced by STR was measured by MTT assay. Methods : Fibroblasts were cultured in the media containing various concentrations of STR for 72 hours. In addition, cytoprotective effect of Drynariase Rhizoma(DR) on STR-induced cytotoxicity in fibroblasts was examined when fibroblasts were preincubated with various concentrations of DR for 2 hours before treatment of 5 ㎍/ml STR for 72 hours. Results : STR decreased remarkably cell viability in a dependentmanner in these cultures, and also DR increased cell viability and amount of DNA synthesis damaged by STR. Conclusions : From the above results, it is suggested that STR has toxic effect in cultured mouse fibroblasts, and also DR was effective in the protection of STR-induced cytotoxicity in these cultures.