방사선조사가 금붕어 장관평활근 수축활성 변화에 미치는 영향 = Effects of Irradiation on Contractility Changes of Smooth Muscles in the Intestine of Goldfish

다국어 초록 (Multilingual Abstract)

Irradiation, which acts directly on human tissue and produces
reactive oxygen radicals by ionizing water molecules, causes
significant morbidity and mortality. Muscle tissue is damaged by
direct action of the radiation and by oxygen radicals produced by
the radiation. The irradiation caused contractility changes in the
smooth muscles in the intestine of goldfish, and those changes were
measured by using a physiograph. A total of 36 healthy goldfish
weighing from 50 to 70 g were divided into nine groups. The control
group was without irradiation. The eight experimental groups were
sacrificed at 2 hours, 1 day, 2 days, 3 days, 4 days, 6 days, 9
days, and 40 days after 10-Gy irradiation.

The results were as follows:

1. The contractility of the control group was about a gravitational
force of 1.15$\pm$0.15 g.

2. The contractility decreased to 0.76$\pm$0.10 g, 0.52$\pm$0.10 g,
0.53$\pm$0.17 g, 0.55$\pm$0.07 g, and 0.67$\pm$0.14 g (p $<$ 0.05)
at 2 hour, 1 day, 2day, 3 day, and 4 day after irradiation,
respectively. Although the contractilities on the 6th day, the 9th
day, and the 40th day were also less than the control
contractilities of 0.92$\pm$0.18 g 1.02$\pm$0.16 g, and
1.07$\pm$0.15 g, respectively, the difference was not statistically
significant (p $>$ 0.05).

3. After irradiation, the contractility decreased with time until
day 3. However, the contractility was observed to increase on day 4
and continued to increase through day 40. Ultimately, the
contractility of the smooth intestinal muscles of goldfish recovered
to normal at a time greater than 40 days following the irradiation.

국문 초록 (Abstract)

방사선조사 시 방사선의 직접적인 작용, 자유산소기의 간접적인 작용에
의해 근육 손상이 일어난다. 방사선조사가 금붕어 장관평활근의
수축활성에 어떤 영향을 나타내는지 알아보고자 본 연구를 시작하였다.
선형가속기를 이용하여 6 MV X-선 10 Gy를 금붕어에 전신조사 후 시간
경과에 따라 4마리씩 총 8개 실험군으로 분류하여, 장관평활근 수축활성
변화를 생리기록기로 각각 3번씩 측정하여 대조군 (Co)과 비교하였다.
방사선조사로 금붕어 장관평활근에 손상이 일어나 근육 미세구조의
변화가 나타났으며, 방사선조사 후 4일, 6일, 9일에는 출혈, 색변성 등
장관평활근의 변화가 관찰되었고 사망하는 개체도 있었다. 방사선조사 후
시간 경과에 따른 금붕어 장관평활근의 수축성 변화는 대조군 (Co)의
장관평활근 수축활성이 1.15$\pm$0.15 (g중)이었던 것이 방사선조사 후
2시간, 1일, 2일, 3일, 4일까지는 각각 0.76$\pm$0.10 (g중),
0.52$\pm$0.10 (g중), 0.53$\pm$0.17 (g중), 0.55$\pm$0.07 (g중),
0.67$\pm$0.14 (g중)으로 수축활성이 50 \% 정도 감소하였다. 방사선조사
후 6일, 9일, 40일에는 금붕어 장관평활근 수축활성이 0.93$\pm$0.18
(g중), 1.02$\pm$0.16 (g중), 1.07$\pm$0.15 (g중)으로 방사선조사 전
보다 수축활성은 감소하였으나 그 정도는 그리 크지 않았다. 결과적으로,
방사선 조사후 4일 이후부터는 손상된 세포가 회복되고 있는 것으로
판단된다.

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