Pityrosporum속에 대한 항진균제의 최저억제 농도 = A Study on the Minimal Inhibitory Concentration of Some Antifungal Agents against Genus Pityrosporum

국문 초록 (Abstract)

Pityrosporum(이하 P)의 표준균주 P. orbiculare ATCC42132, P. ovale ATCC14521, P. pachydermatis ATCC14522 3개균주와 전풍 및 지루성 피부염환자에서 분리된 P. orbiculare 6개균주 그리고 정상인에서 분리된 P. orbiculare 3개균주, P. ovale 3개균주 총 15개균주에 대한 전신요법 항진균제 amphotericin-B, ketoconazole, itraconazole과 국소요법제 clotrimazole, sodium thiosulfate, selenium sulfide의 MIC를 측정하였다.
P에 대한 전신요법제 amphotericin-B, ketoconazole, itraconazole의 MIC는 각각 0.01-25.6㎍/㎖, 0.01-3.2㎍/㎖, 0.05-1.6㎍/㎖로 itraconazole이 가장 낮았고, 국소요법제 clotrimazole, sodium thiosulfate, selenium sulfide의 MIC는 각각 0.2-320㎍/㎖, 1-200㎎/㎖, 0.02-0.64㎎/㎖로 clotrimazole이 가장 낮았다.
P. orbiculare에 대한 MIC는 전신요법제로는 itraconazole이 0.05-1.6㎍/㎖로 가장 낮았고, 국소요법제는 clotrimazole이 0.2-320㎍/㎖로 가장 낮았다. P. ovale에 대한 MIC는 전신요법제로는 역시 itraconazole이 0.1-0.2㎍/㎖로 가장 낮았고, 국소요법제로는 selenium sulfide가 0.02-0.04㎎/㎖로 가장 낮았으며, 전체적으로는 P. ovale가 P. orbiculare 보다 약제에 더욱 민감한 것으로 나타났다.
전풍 및 지루 피부염 환자에서 분리된 P에 대한 MIC는 전신요법제의 경우로 두질환 모두에서 itraconazole이 각각 0.2-1.6㎍/㎖, 0.2-0.4㎍/㎖로 가장 낮았고, 국소요법제로는 전풍환자에서는 clotrimazole과 selenium sulfide가 각각 2.5-320㎍/㎖, 0.02-0.32㎎/㎖로 가장 낮았고, 지루성피부염 환자는 clotrimazole이 0.2-5㎍/㎖로 가장 낮았으며, 지루 피부염에서 분리된 P가 전풍 환자에서 분리된 P보다 약제에 더욱 민감한 것으로 나타났다.

다국어 초록 (Multilingual Abstract)

The auther investigated the minimal inhibitory concentration (MIC) of some antifungal agents against Genus Pityrosporum.
The tested strains were a total of 15 in number. They were 3 standard strains of Pityrosporum (P. orbiculare ATCC42132, P. ovale ATCC14521, P. pachydermatis ATCC14522), 3 of P. orbiculare from patients with tinea versicolor, 3 of P. orbiculare from seborrheic dermatitis, 3 of P. orbiculare and 3 of P. ovale from normal individuals. The tested antifungal agents were amphotericin-B, ketoconazole, itraconazole, clotrimazole, sodium thiosulfate, selenium sulfide. A various concentration of drugs were mixed into media containing distilled water, neopeptone, agar, dextrose, yeast extract, olive oil, and Tween 80. The inoculum contained 20,000-40,000 colony forming units(cfu) of Pityrosporum,
The results were as follows :
1. MIC ranges of systemic amphotericin-B, ketoconazole and itraconazole were 0.01-25.6 ㎍/㎖, 0.01-3.2 ㎍/㎖ and 0.05-1.6 ㎍/㎖, respectively. Itraconazole showed the lowest value.
2. MIC ranges of topical clotrimazole, sodium thiosulfate and selenium sulfide were 0.2-320 ㎍/㎖, 1-200㎎/㎖ and 0.02-0.64㎎/㎖, respectively. Ketoconazole cream showed the lowest value.
3. The lowest values of MIC of antifungal agents against P. orbiculare were 0.05-1.6 ㎍/㎖ for systemic itraconazole, 0.2-320㎍/㎖ for clotrimazole. Those against P. ovale were 0.1-0.2 ㎍/㎖ for itraconzole, 0.02-0.04㎎/㎖ for selenium sulfide. P. ovale, as a whole, was more sensitive than P. orbiculare.
4. The lowest values of MIC against Pityrosporum form the lesions of tinea versicolor were 0.2-1.6㎍/㎖ for itraconazole, 2.5-320㎍/㎖ for clotrimazole, 0.02-0.32㎎/㎖ for selenium sulfide. Those against Pityrosporum from the lesions of seborrheic dermatitis were 0.2-0.4㎍/㎖ for itraconazole, 0.2-5㎍/㎖ for clotrimazole. Pityrosporum from the lesions of seborrheic dermatitis, as a whole, was more sensitive than Pityrosporum from tinea versicolor.