농어목 경골어류 유문수의 장내분비 세포에 대한 면역세포화학적 연구 = Immunocytochemical study on the enteroendocrine cells of the pyloric caecum in fourteen species of teleostean percida

국문 초록 (Abstract)

농어목 쑤기미, 쥐노래미, 노래미, 종대우럭, 방어, 감성돔, 참돔, 돌돔, 뱅어돔, 볼낙, 등가시치, 풀망둑, 농어 및 능성어 유문수 점막에 출현하는 장내분비세포 중 somatostatin, serotonin, gastrin, PP, CCK-8 및 glucagon 세포들을 면역세포화학적 방법으로 염색한 후 광학현미경하에서 이들 세포의 분포, 출현빈도, 모양 및 분비형태를 비교 관찰하였다.
방어, 등가시치 및 풀망둑은 모든 장내분비세포가 관찰되지 않았고 장내분비세포들 중 somatostatin 및 glucagon 세포는 모든 종에서 관찰되지 않았으며 그 외 장내분비세포들의 출현은 어종에 따라 차이가 있었고 능성어에서 가장 풍부하였다.
능성어는 serotonin, gastrin, PP, 및 CCK-8을 분비하는 4 종류 세포들이, 종대우럭, 감성돔, 참돔은 gastrin, PP, 및 CCK-8을 분비하는 3 종류의 세포들이, 그리고 쥐노래미, 돌돔 및 벵어돔은 gastrin 및 PP을, 쑤기미와 노래미는 gastrin과 CCK-8을, 농어는 PP와 CCK-8을 분비하는 2 종류의 세포가 각각 관찰되었다.
Gastrin 세포는 쥐노래미, 능성어 및 종대우럭은 중등도 출현하였으며 그 외 종에서는 소수 출현하였다. PP 세포는 농어에서 다수, 능성어에서 중등도, 벵어돔에서 극소수 그 외종에서는 소수 관찰되었다. CCK-8 세포는 능성어에서 중등도, 그 외종에서는 소수 출현하였으며, serotonin 세포는 능성어에서만 극소수 관찰되었다.
장내분비세포는 어종에 따라 유문수에 개방형 장내분비세포와 폐쇄형 내분비세포가 나타났으며, gastrin 세포는 종대우럭, 볼낙 및 능성어는 폐쇄형만 존재하고 기외종에서는 두 형이 공존하나 쥐노래미와 감성돔은 폐쇄형이 더 많고 노래미, 참돔 및 벵어돔은 개구형이 더 많았으며 쑤기미과 돌돔은 두 형이 비슷하게 섞여 있었다.
PP세포는 농어, 능성어, 벵어돔, 종대우럭 및 돌돔은 폐쇄형이었으며 쥐노래미는 개구형이었으나 참돔과 감성돔은 개구형과 폐쇄형이 섞여 있었고 참돔에서는 개구형이, 감성돔에서는 폐쇄형이 각각 더 많았다. CCK-8 세포는 능성어, 농어 및 종대우럭은 폐쇄형이었고, 참돔은 개구형이었으나 그 외 종에서는 개구형과 폐쇄형이 섞여 있었으며 노래미에 개구형이 더 많았고, 쑤기미와 감성돔은 두 형이 비슷하였다. serotonin 세포는 능성어에서 개구형과 폐쇄형이 섞여 있었다.

다국어 초록 (Multilingual Abstract)

The immunocytochemical properties of the somatostatin, gastrin, PP, CCK-8, and glucagon cells in the pyloric caeca, fourteen species of percida were used; Inimicus japonicus, Hexagrammos otakii, Agrammus agrammus, Epinepbelus latifasciatus, Lateloabrax japonicus, Epinepbelus septemfasciatus, Seriola quinqueradiata, Sparus swinbonis, Pagrosomus major, oplegnathus fasciatus, Girella punctata, Sebastes inermis, Zoarces gillii and Acantbogobius basta.
For the demostration of six enteroendocrine cell types of the pyloric caecum, the peroxidase-anti peroxidase(PAP) method with each antisera was used.
The frequency of entroendocrine cells per unit area(㎟)of the pyloric caecal mucosa was counted, and the shape and distribution pattern of the cells were observed.
None of the enteroendocrine cells were detected in the pyloric caec of the three species; Seriola quinqueradiata, Zoarces gillii, and Acantbogobius basta. Especially, two enteroendocrine cell type such as somatostatin and glucagon cells were not detected in all species. In the distribution pattern and the frequency of the remaining three enteroendoctine cells were differed depending on the species, but the frequency of these cells were abundantly in Epinepbelus septemfasciatus.
In the pyloric caeca, Epinepbelus septemfasciatus, four endocrine cell types, such as serotonin, gastrin, PP, and CCK-8, Epinepbelus latifasiatus, Sparus swinbonis and Pagrosomus major, three endocrine cell types, such as gastrin, PP, and CCK-8, Hexagrammos otakii, oplegnatbus fasciatus, and Girella punctata, two endocrine cell types, such as gastrin and CCK-8, inimicus japonicus and Agrammus agrammus, two cell types, such as gastrin and CCK-8, Lateloabrax japonicus, two endocrine cell types, such as PP and CCK-8 were observed, respectively.
The gastrin cells of all species investigated were distributed a moderate number in Hexagrammos otaki, Lateloabrax japonicus and Epinepbelus latifasiatus, while these cells were occurred a small number in the remaining species. The PP cells of all species inverstigated were appeared a large number in Lateloabrax japonicus, a moderate number in Epinepbelus septemfasciatus, a few number in Girella punctata, and a small number in the remaining species, respectively. The CCK-8 cells of cell species investigated were distributed a moderate number in Epinepbelus septemfasciatus, while were occurred a small number in the remaining species. The serotonin cells were showed a few number in Epinepbelus septemfasciatus only.
The enteroendocrine cells of the pyloric caecum have been recognized two main types, such as open and closed types depending on the species. The gastrin cells of Epinepbelus latifasiatus. Sebastes inermis and Epinepbelus septemfasciatus were observed only closed type, but these cells of the remaining species were detected in both types. Especially, the closed type of these cells were prominent in Agrammus agrammus, Pagrosomus major, and Girella punctata. The shape of PP cells was appeared open type in Hexagrammos otakii, but these cells were detected closed type in the remaining species except Pagrosomus major and Sparus swinbonis. The shape of CCK-8 cells were closed type in Epinepbelus septemfasciatus, Latesoabrax japonicus and Epinepbelus lactifasiatus, and open type in Pagrosomus major, but these cells of the remaining species were detected in both types. Especially, the open type of these cells were dominant in Agrammus agrammus, the two types cells of Inimicus japonicus and Sparus swinbonis were similarly detected. The two types of serotonin cells were mixed in Epinepbelus septemfasciatus,

목차 (Table of Contents)

• 目次
• Ⅰ. 서론
• Ⅱ. 재료 및 방법
• Ⅲ. 결과
• Ⅳ. 고찰
• Ⅴ. 요약
• 참고 문헌
• Abstract