타액피막에 형성된 글루캔에의 Streptococcus mutans의 부착 : 열과 화학제제에 의한 세균과 피막의 전처리 영향 Pretreatment effects of bacteria and pellicle by heat and chemicals = Study on the adherence of Streptococcus mutans to glucans formed in situ in salivary pellicle

다국어 초록 (Multilingual Abstract)

Based on the report that the adherence of S. mutans 10449 was enhanced by the presence of glucan formed in situ on the salivary pellicle, the present experiment was carried out to elucidate the role that glucans play in the enhancement. It was asked whether the gluans are involved directly, or indrectly by affecting other adhesion-related factors. In addition, the property of the adhesin which responds to the in situ formed gluan was examined.
Glucosyltransferase(GTF) was prepared by ammonium sulfate fractionation of S. mutans 6715-7 culture supernatant. Saliva-coated hydroxyapatite beads(SHA) were treated with GTF, then incubated with 5% sucrose to allow in situ glucan synthesis(SHA/glucan). The pellicles or bacterial suspensions were treated with heat, trypsin or periodate. For the bacterial adherence assay, SHA or SHA/glucan was combined in polypropylene microfuge tubes with [^3H]-thymidine labeled S. mutans 10449 and incubated. The beads were then washed and counted with scintillation fluid to determine the number of adherent bacteria.
Although the pretreatment of pellicles with heat or trypsin reduced significantly the adhesion of S. mutans 10449 to SHA/glucan, periodate pretreatment caused the most reduction, implying that the glucan is directly involved in the bacterial adhesion. When the bacteria were pretreated with heat or trypsin, the latter influenced the adhesion mostly. This suggests that the bacterial adhesin involved is of protein-nature.

목차 (Table of Contents)

• Ⅰ. 서 론
• Ⅱ. 재료와 방법
• Ⅲ. 성적 및 고찰
• Ⅳ. 결 론
• 참고문헌
• 영문초록