Ethionine 및 Adenine이 간세포 Cytosome의 Acid Phosphatase 분포에 미치는 영향에 관한 전자현미경적 연구 = An Electron Microscopie Study of the Effects of Ethionine and Adenine to the Acid Phosphatase Distribution in the Cytosome of Hepatocytes

국문 초록 (Abstract)

잡종 흰쥐에 etionine으로 간세포의 변성을 유도하고, 또 ethionine으로 변성을 일으킨 간세포를 adenine으로 회복 시켰을때 간세포에 나타난 acid phasphatase의 분포를 조사하고 얻은 성적을 요약하면 다음과 같다.
Ethionine은 간의 상해를 유도하여, autophago cytosis를 증가 시키나, 이를 처리하기 위한 primary lysosme의 형성은 상당히 저해되었다. 그러나 ethionine 투여후 adenine을 투여한 군에서는 autophagocytosis는 ehionine단독 투여군보다 경미한 반면, primary lysosome은 현저히 증가하였다.
이로 미루어보아 ethionine으로 야기된 간세포의 상해시에는 단백 합성과 ATP의 생산 장애로 인하여 파괴내지 변성된 세포내 소기관의 처리에 주역을 담당하는 primary lysosome의 형성부전으로 많은 파괴산물이 세포내에 잔류하나 adenine으로 이를 경감시키면 primary lysosome의 생성이 왕성해져서 파괴산물의 처리가 신속히 되는 것으로 사료된다.

다국어 초록 (Multilingual Abstract)

It is well known that ethionine induces degenerative changes of liver cells and adenine may reverse or prevent its cytotoxic properties. Most of injured cells, as King indicated, are degenerated and destroyed, following loss of their metabolic equiribrium. during these process, degenerated cytoplasmic organelles are destroyed by the process known as "autophagocytosis". It is certain that lysosome intimately participated in this process, but confusions were existed in the term "lysosome, and the relation of autophagocytosis, digestion of ingested foreign material and the role of lysosome in these process is still uncertain. Arstilla claimed that the membrane of autophagic vacuole is orginated from smooth surfaced endoplasmic reticulum and these structures are fused with primary lysosome orginated from Golgi complex, which facilitate digestion of degenerated organells.
What stimulates autophagocytosis? What happen during recovery from degeneration? In an effort to clarify the relationships between autophagic vacuole and primary lysosome in a respect digestion of degenerated organelles, particulary during degeneration and recovery from it, the author tried to study the distrbution of acid phosphatase activity in rat liver cells during degeneration and recovery from it.
DL-Ethionine(l mg/gm of body weight) was injected intraperitoneally to 24 rats and adenine sulfate(32 mg a animal) was injected intraperitoneally to the half of them 6 hours after initial injection. Control groups were received salin only, ethionine and saline, or saline and adenine respectively. Each group of animals was killed at 3,6,9, and 12 hours after the last injection respectively and the livers were taken from the rats. Sections were incubated in solution of beta-blycerphosphate at 20°-25℃ for 30-40 mimutes and observed by the light and electron imcroscopes.
The results obtaine were as follows:
Acid phosphatase activity was found in the autophagic vacuoles or primary lysosome after ethionine injection with increase of number of lysosome and autophagic vacuoles, but it am ounts were not increased after adenine injection. Acid phophatase reaction deposits was found in the lumen of endoplasmic reticulum and in the lysosome andautophagic vacuoles. Ethionine increased autophagocytosis in the liver cells but formation of primary lysosomes was considerably inhibited. In the groups treated with adenine after ethionine injection, autophagocytosis was also increased but milder than groups which were injected ethionine only, altoough increase of primry lysosome formation was prominent.
The results suggest that ethionine indouced-inhibition of ATP and protein synthesis causes derangement of formation of primary lpsosome, which plays a main role in the scavenging of degenerated or destructed organelles, but adenine recovers ATP and protein synthesis, which facilitates formation of primary lysosome so that the rate of scavenging of destructed organelles is accelerated.