자궁내막조직중 PAPP-A분포에 관한 면역효소 조직화학적 연구 = An Immunoenzyme Histochemical Study on Localization of Pregnancy-Associated Plasma Protein-A in Human Endometrial Tissue

다국어 초록 (Multilingual Abstract)

Pregnancy associated plasma protein A (PAPP-A) which was supposed to be specific to placenta is now known to be distributed in normal and various pathologic endometrial tissue. This distribution of P-4PP-A in endometrial tissue was detected by solid-phase radioimmunoassay from tissue extract. Therefore, to understand exact localization of PAPP-A among various tissue component of the endometrial tissue, the author collected formalin fixed, paraffin embedded surgical specimens of normal and pathologic endometrial tissue. Paraffin sections of the specimens were treated by immunoenzyme histologic technic of avidinbiotin complex method using biotinylated rabbit anti-human PAPP-A.
The result obtained are as follows;
1. Glandular epithelium of normal endometrium revealed positive reaction to anti PAPP-A antibody only during secretory phase of the menstrual cycle. However, stroma of normal endometrium showed persistent positive reaction to anti PAPP-A antibody throughout the cycle.
2. Predecidual cells exhibited strong positive reaction to anti-PAPP-A antibody.
3. Reaction of epithelium and stroma in cystic hyperplasia of endometrium to anti PAPP-A was similar with that of normal endometrium in secretory phase of menstrual cycle showing positive reaction in both components.
4. Reactions of epithelium and stroma in adenomatous hyperplasia of endometrium, endometrial polyp and epithelium of endometrial adenocarcinoma to anti PAPP-A were similar with that of normal eidometrium in proliferative phase showing negative reaction in epithelium and positive reaction in stroma.
Above findings suggest that pathologic change of the endometrium is associated with loss of reactivity to anti-PAPP-A in endometrial stroma.