巴豆加大黃이 抗腫瘍作用과 自然殺害細胞의 活性에 미치는 영향에 대한 실험적 연구

다국어 초록 (Multilingual Abstract)

This experiment was designed to study the antitumor effats ad Activity of Natura Killer Cell of semen Tighi plus Rhizoma Rhei.
The cytotoxic and antitumor effects were evaluated on human cell lines(A549, Caki-1, LL2, Sarcomal8O, NIH/3T3) after expsure to prebrewed Semen Tiglii plus Rhizoma Rhei water extract 0.l, 0.2, 0.4, 0.8, 1.6mg/ml using in MTT assay. LDH, colony forming efficency and SRB assay which were regarded as a valuable method for cytotoxic and antitumor effects of unknown compound on tumor cell lines.
The results obtained in this studies were as follows.
l From the rsult of MTT assay, the cytotoxicity of ST(生巴豆霜), ST+RR(生巴豆霜加大黃) were concentration-de*ndently increased in both group of the ST and ST+RR, the cytotoxicity of ST+RR(生巴豆霜加大黃) Was Similar to that of ST(生巴豆霜).
2 From the result of LDH, the cytotoxicity of ST, ST +RR were concentrati -on-dependently increased in both group of the ST ad ST+RR, the cytotoxicity of ST+RR was Simla to that of ST.
3. The antitumor effect on A549 tumor cell from the result of colony foming efficiency showed the inhibitory effect on the growth in both group of the ST and ST+RR, the inhibitory effect on growth was low slightly in the ST+RR.
4. From thc result of SRB assay. the antitumor effect on caki-l tumor cell of ST. ST+RR showed the inhibitory effect on the growth in both group of the ST and ST+RR, the antitumor effect of ST+RR was similar to that of ST.
5. Nedian survival time and increased life span were increased slightly in both group of the ST and ST+RR.
6 The inhibitory effect on the growth of Sarcoma l80 and Lewis lung carcinoma tumor cell were increased slightly in both group of thc ST and ST+RR.
7. The itchyity of NK cell was increased in the ST+RR.