Description of <i>Lutimonas</i> <i>halocynthiae</i> sp. nov., isolated from a golden sea squirt (<i>Halocynthia aurantium</i>), reclassification of <i>Aestuariicola saemankumensis</i> as <i>Lutimonas</i> <i>saemankumensis</i> comb. nov. and emended descr

다국어 초록 (Multilingual Abstract)

<P>A Gram-stain-negative, non-motile, coccoid, ovoid or rod-shaped bacterial strain, designated RSS3-C1<SUP>T</SUP>, was isolated from a golden sea squirt (<I>Halocynthia aurantium</I>) collected from the East Sea, South Korea. Strain RSS3-C1<SUP>T</SUP> was found to grow optimally at 20–25 °C, at pH 7.0–8.0 and in the presence of 2.0 % (w/v) NaCl. Phylogenetic trees based on 16S rRNA gene sequences revealed that strain RSS3-C1<SUP>T</SUP> clustered with the type strains of <I>Lutimonas vermicola</I> and <I>Aestuariicola saemankumensis</I>. Strain RSS3-C1<SUP>T</SUP> exhibited 98.8 % 16S rRNA gene sequence similarity to each type strain. Strain RSS3-C1<SUP>T</SUP> contained MK-6 as the predominant menaquinone and iso-C<SUB>15 : 0</SUB>, iso-C<SUB>17 : 0</SUB> 3-OH and anteiso-C<SUB>15 : 0</SUB> as the major fatty acids. The major polar lipids of strain RSS3-C1<SUP>T</SUP> were phosphatidylethanolamine and two unidentified lipids. The DNA G+C content of strain RSS3-C1<SUP>T</SUP> was 39.2 mol%, and DNA–DNA relatedness to the type strains of http://dx.doi.org/10.1601/nm.11271 and http://dx.doi.org/10.1601/nm.13687 was 21±5.3 and 26±7.5 %, respectively. The differential phenotypic properties, together with its phylogenetic and genetic distinctiveness, revealed that strain RSS3-C1<SUP>T</SUP> is separated from http://dx.doi.org/10.1601/nm.11271 and http://dx.doi.org/10.1601/nm.13687. On the basis of the data presented, strain RSS3-C1<SUP>T</SUP> is considered to represent a novel species of the genus <I>Lutimonas</I>, for which the name <I>Lutimonas halocynthiae</I> sp. nov. is proposed. The type strain is RSS3-C1<SUP>T</SUP> ( = KCTC 32537<SUP>T</SUP> = CECT 8444<SUP>T</SUP>). In this study, it is also proposed that <I>Aestuariicola saemankumensis</I> should be reclassified as a member of the genus <I>Lutimonas</I>, as <I>Lutimonas</I> <I>saemankumensis</I> comb. nov. (type strain SMK-142<SUP>T</SUP> = KCTC 22171<SUP>T</SUP> = CCUG 55329<SUP>T</SUP>), and the description of the genus <I>Lutimonas</I> is emended.</P>