Bacillus subtilis var. 816의 Phage에 關한 硏究 = A Study on the bacteriophage of Bacillus subtilis var. 816

국문 초록 (Abstract)

1. Bacillus subtilis var. 816의 異常醱酵는 phage L의 感染에 依한 것이었다.
2. phage L은 毒性 phage로서 plaque는 中央이 透明한 圓形이며 直徑이 1±0.1mm의 大型 plaque와 0.3∼0.5mm의 小型 plaque가 나타났다. 小型 plaque는 phage L에 依해 prophage가 誘發된 溶原 phage의 plaque로 思料된다.
3. phage L₁의 形態는 直徑 102.3nm의 頭部와 170.4nm의 尾部 그리고 20.4nm의 end plate가 있고 phage L₂는 尾?(초)가 100nm로 收縮되었고 end plate 밑으로 80nm의 tube가 나와있다. phage L₁과 L₂는 同種의 phage로서 phage L₂는 核酸을 放出한 粒子이다.
4. phage L은 pH 5.0∼7.0까지 安定性을 나타냈다.
5. phage L은 40℃까지 安定性을 나타냈고 60℃에서 완전히 不活性化된다.
6. moi 1로 感染된 宿主菌은 4時間後에 溶菌된다.

다국어 초록 (Multilingual Abstract)

1. A phage was isolated from the strain of Bacills subtilis var. 816 that brought about irregular soybean fermentation and it was designated phage L.
2. The phage L was virulent phage under experimental condition. It produced round plaques with clear center. The mean size of plaques were 1 ±0.1 mm in diameter. Large and small plaques revealed in the same plates in spite of isolation from single large plaque. The small plaque seemed to be temperate phage that induced from prophage by that virulent phage L infected.
3. Electronmicroscopy showed phage L₁and L₂had a same size (102.3nm in diameter) and multilateral head but the parts of tail were different between phage L₁and L₂. Phage L₁had extended sheath (170.4nm)and end plate (20.4nm), but the sheath of phage L₂contracted to the 61 nm and a tube was attached under end plate. The phage L₂probably was DNA-less ghost after injection.
4. The phage L was stable at the range of pH 5.0∼7.0 in nutrient broth.
5. Thermostability experiments indicated that phage L was stable until 40℃ in nutrient broth. However the infectivity of the phage L was completely inactivated at 60℃ for 30 min.
6. In case that phage L was infected to m.o.i. 1, the host bacteria was lysed after 4 hours.