실험적 허혈성 급성 신부전에서 칼슘 길항제의 투여가 신장과 혈장 Renin 활성도에 미치는 영향 = The Effect of Systemic Nifedipine Pretreatment on Renal Function & Plasma Renin Activity in Experimental Ischemic Acute Renal Failure

국문 초록 (Abstract)

허혈성 급성신부전이 예상되는 경우, 칼슘대사 길항제인 Nifedipine을 전처치하여, 신기능 감소의 예방효과 및 혈장 Renin 활성도의 변화를 관찰하기 위하여, 고양이 10마리를 대조군, 실험군으로 나누어 실험 연구한 결과, 통계학적으로 유의하지는 않으나, 신기능 감소정도를 둔화시키며, 혈장 Renin 활성도의 변화율을 감소시키는 것으로 추정되었다.

다국어 초록 (Multilingual Abstract)

In the process of ischemic cell injury, alterations in one such variable, that of cellular calcium homeostasis, appear to be of major significance. Probably calcium entry blockers could protect against, attenuate the degree of, or enhance recovery from, renal ischemic injury by several mechanisms.
And the retrain-angiotensin system may play a central role in the pathogeneisis of acute renal failure(ARF). The evidence that cytosolic Ca2+ is an inhibitory second messenger in renin secretion has been reviewed recently.
Therefore the purpose of this study was to evaluate the effect of systemic calcium entry blockers pretreatment on renal function & plasma retrain activity (PRA) in the experimental ischemic ARF model.
Ten cats were anesthesized with pentobarbital sodium (40mg/kg, I.M) and a tracheostomy, two IV line and a urinary catheter were placed In position. Temperature was maintained at 37.5 degrees C. By an abdominal approach, both renal arteries were isolated. Five cats were used as controls and received saline for 2 hours before bilateral renal artery clamp. Another five cats were treated with nifedipine (10mg/kg) subcutaneously 2 hours before bilateral renal artery clamp. All ten cats underwent 1 hour of renal artery clamp followed by 3 hours of reperfusion and hydration with saline. Before clamp and after reperfusion, blood and urine were sampled for creatinine, Na, β2-microglobulin, PRA and urine volume was measured.
The resuls were as follows : In the control group, the Ccr value was 125.5±151.6ml/min/kg before clamp and decreased to 6.2±5.3ml/min/kg after reperfusion; in the experimental group, the Ccr value was 43.9±48.1ml/min/kg before clamp and decreased 5.6±5.9ml/min/kg after reperfusion(p<0.05).
And creatinine clearance decreased experimental group(88.9±7.9%) compared with control group(98.7±11.1%), these results were not significant statistically(p>0.05). In the control group the PRA value was 14.9±9.2ng/ml/hr before clamp and decreased 14.0±10.1ng/ml/hr after reperfusion; in the experimental group the PRA value was 11.5±6.9ng/ml/hr before clamp and decreased 10.3±3.7ng/ml/hr after reperfusion(p>0.05).
From these data, it was suggested that systemic nifedipine pretreatment exerts the insignificant protective effect on ARF and influence on the decrement value of PRA insignificantly.

목차 (Table of Contents)

• Ⅰ.서론
• Ⅱ.실험동물 및 방법
• 1.실험동물
• 2.실험방법
• Ⅲ.연구성적
• 1.허혈전 신기능
• 2.허혈후 신기능
• 3.허혈 전후 신기능의 비교
• 4.혈장 Renin 활성도(Plasma renin acitvity : PRA)의 변화
• Ⅳ.고안
• Ⅴ.결론