자색옥수수 포엽 추출물의 지표성분 Cyanidin-3-O-Glucoside 분석법 유효성 검증 = Validation of the HPLC Analysis Method for Cyanidin-3-O-Glucoside as a Marker Compound of Purple Corn Husk Extract

다국어 초록 (Multilingual Abstract)

Cyanidin-3-O-glucoside (C3G) was selected as a marker compound to standardize the husk extract of purple corn for potential applications in food and functional ingredient development. An analytical method for C3G was established and validated using high-performance liquid chromatography with photodiode array detection (HPLC-PDA). The method showed specificity because the retention time and spectrum of the C3G standard solution matched those of the purple corn husk extract. The method showed high specificity because the retention time and UV spectrum of the C3G standard solution were consistent with those of the purple corn husk extract. The calibration curve showed excellent linearity with a correlation coefficient (R²) greater than 0.9999. The limits of detection (LOD) and quantification (LOQ) were 0.065 μg/mL and 0.197 μg/mL, respectively. Intra-day analysis revealed accuracies ranging from 91.73% to 101.06% and precision with relative standard deviations (RSDs) of 2.54%-3.96%. Inter-day analysis showed accuracies between 96.89% and 99.25% with RSDs of 2.20%-3.53%. The recovery rates of C3G at different concentrations ranged from 97.78% to 100.94%, with RSDs below 1%. These results suggest that the established HPLC method is reliable and suitable for the quantitative analysis and standardization of C3G in purple corn husk extract.