사염화탄소 장기투여로 초래된 간 세포의 핵변화에 관한 전자 현미경적 연구 = Electron Microscopic Study on the Nuclear Changes After Long-Term Carbon Tetrachloride Administration

다국어 초록 (Multilingual Abstract)

The author has carried out light and electron microscopic investigation to delineate the intriguing nuclear modifications of hepatocyte after long-term exposure to carbon tetrachloride.
The female Sprague-Dawley albino rats were received subcutaneous injections, twice a week for thirteen weeks, of 0.13㎖. 50 per cent solution of carbon tetrachloride in olive oil per 100㎎ of body weight. In each instance, the rats were sacrificed three days after the last injection. The control rats were not received any treatment.
The results obtained are as follow:
1. The author's findings on fine structure of nucleus in hepatocyte of normal rats are almost similar to that of other investigators.
2. The morphological effects of long term administration of carbon tetrachloride upon the nucleus of hepatocyte are summerized as below.
a)Two types of morphologically altered hepatocytes are encountered. One of these, corresponding to slightly basophilic cell in the H-E preparation, is characterized by proliferation of rough-surfaced endoplasmic reticulum. And the other is characterized by irregularly arranged, dilated and reduced in amount of rough-surfaced endoplasmic reticulum which is corresponded to esinophilic and lipid containing cell in the H-E preparation. However, the nuclear pattern observed in the two types of these cells are, in some respect, similar.
b) The nucleus are usually enlarged and chromatin material is more clumped then in the controls tending to be marginated near the perinuclear membrane.
c) The nuclear pores show prominant and numerous then are evident in control one.
d) The nucleoli of the slightly basophilic cell are relatively large and occasionally multiple.
e) The space delimited by the perinuclear membrane is often widened in lipid containing cells. and such nuclei frequently contain inclusions. The inclusions display multiple vesicle, lipid bodies or mitochondria-like figures which are delimited from the nuclear substance by a distinct single or double-layered membranes.
f) These inclusions are thought to be as investigation of cytoplasmic components.
3. the nuclear modifications following longterm carbon tetrachloride administration are believed due to secondary to cytoplasmic derangement, which suggest active participation of nuclei in cytoplasmic modification found after prolonged exposure to carbon tetrachloride.