병귤과피의 주정 침출 중 유용성분의 변화 = Changes of Major Constituents by Soaking of Citrus platymamma Peel with Spirit Solution

다국어 초록 (Multilingual Abstract)

In order to prepare liqueur of Citrus platymamma, 500 g dried peel was soaked in 10 l (5%, w/v) of $30{\sim}95%$ ethanol concentration for 70 days. Changes in pH was $5.19{\sim}4.80$ with 30% ethanol concentration, and the pH was decreased as ethanol concentration decreases for 10 days after soaking. Color a-value was decreased and color b-value was increased according to higher ethanol concentration. Extract was $2.00{\sim}2.19%$ (w/v) with $30{\sim}70%$ ethanol concentration. Acid content was $0.18{\sim}0.21%$ (v/v) with $30{\sim}70%$ ethanol concentration, while $0.13{\sim}0.15%$ (v/v) with 95% ethanol concentration. The contents of fructose and glucose was increased with lower ethanol concentration, and sucrose content was decreased with longer soaking time. Main flavonoids were narirutin, hesperidin, nobiletin, 3,5,6,7,8,3'4'-methoxylated flavone, and tangeretin. Most flavonoids were extracted more than 80% of ethanol for $3{\sim}5$ days soaking. Total polyphenol was $628.8{\sim}711.2$ ${\mu}g/ml$ with $30{\sim}70%$ ethanol concentration for 20 days soaking. Therefore, to prepare Citrus platymamma peel liqueur, it is necessary to soak the material in $50{\sim}70%$ ethanol concentration for 20 days.

국문 초록 (Abstract)

제주산 병귤을 침출주의 소재로 활용하기 위하여 $30{\sim}95%$ 주정농도에 건조과피를 각각 500 g/10 l(5%, w/v) 비율로 첨가하여 70일간 침출특성을 검토하였다. 주정농도 30%에서 pH는 $5.19{\sim}4.80$으로 가장 낮았으며, 침출 후 10일까지 주정농도가 낮을수록 감소하는 경향이었다. 주정농도가 높을수록 색도 a값이 낮아지고, b값이 증가하였다. 가용성고형물은 주정농도 $30{\sim}70%$에서 $2.00{\sim}2.19%(w/v)$로, 지속적으로 증가하는 경향이었다. 산함량은 주정농도 $30{\sim}70%$에서 $0.18{\sim}0.21%(v/v)$로 차이가 거의 없었으며, 주정농도 95%에서는 $0.13{\sim}0.15%(v/v)$였다. 주정농도 낮을수록 fructose와 glucose가 많았으며, 침출기간이 길어질수록 sucrose 함량은 낮았다. 병귤과피의 주요 flavonoid는 narirutin, hesperidin, nobiletin, 3,5,6,7,8,3'4'-methoxylated flavone, tangeretin이었고, 대부분의 flavonoids는 침출 후 $3{\sim}5$일에 80%이상 용출되었다. 총 폴리페놀 함량은 주정농도 $30{\sim}70%$로 20일 침출에서 $628.8{\sim}711.2$ ${\mu}g/ml$였으며, 주정원액에서 가장 높은 함량을 나타내었다. 따라서 병귤과피를 침출주 원료로 사용하였을 때, 주정농도 $50{\sim}70%$에서 20일 동안 침출시키는 방법이 효과적이었다.

참고문헌 (Reference)

• 1 Son, "purification and hypotensive effect of bioflavonoidsin Citrus sinensis. J. Korean Soc. Food Nutr. 21" 136-142, 1992
• 2 Laura, B, "Polyphenols: Chemistry, dietary sources, metabolism and nutritional significance provides a nice overview of the nutritional significance of flavonoids as well as other types of food polyphenols" 56 : 317-333, 1998
• 3 Research Institute of National Tex Service, "In textbook of alcoholic beverage-making" Research Institute of National Tex Service 1995
• 4 Kawa, K, "Hesperidin as an inhibitor of lipases from porcine pancreas and Pseudomonas" 61 : 102-104, 1997
• 5 Song, E. Y, "Free sugar, organic acid, hesperidin, naringin and inorganic elements changes of Cheju citrus fruits according to harvest date" 30 : 306-312, 1998
• 6 K. N, "Folin-Ciocalteu reagent for the estimation of siderochromes.Anal. Biochem. 12" 106-112, 1965
• 7 Cha, J. Y, "Effects of hesperitin and naringenin on lipid concentration in orotic acid treated mice" 9 : 389-394, 1999
• 8 Sohn, J. S, "Effect of hesperidin and naringin on antioxidative capacity in the rat" 31 : 687-696, 1998
• 9 Arzouyan C. G, "Differentiation of citrus juices by factorial discriminantanalysis using liquid chromatography of flavanone glycosides.J. Agric. Food Chem. 42" 70-79, 1994
• 10 Rhyu, M. R, "Contents of naringin, hesperidin and neohesperidin in premature Korea citrus fruits" 34 : 132-135, 2002
• 11 Koh, J. S, "Citrus Industry" Jeju-Munhwasa, Korea 163-172, 2001
• 12 Kim, Y. C, "Changes of flavonoids in Jeju native citrus fruits during maturation" 10 : 483-487, 2001
• 13 Kim, B. J, "Carotenoid, color, value, UV spectrum, organic acid and free sugar contents of citrus varieties produced in Cheju" 3 : 23-32, 1996
• 14 Catherine, F, "Bioavailability of the flavanone naringenin and its glycosides in rats" 279 : G1148-G1154, 2000
• 15 Chio, C. S, "Antioxidative activities of Castarea Crenata Flos. methanol extract" 35 : 1216-1220, 2003
• 16 Blois, "Antioxidant activity determination by theuse of a stable free radical. Nature 181" 1190-1200, 1958
• 17 Koh, J. S, "Alcoholic beverages of Jeju" Jeju-Munhwasa, Korea 2003