Testosterone 投與에 依한 核酸含量과 Alkaline Phosphatase 活性變動에 關한 硏究 = A Study on Changes of Alkaline Phosphatase Activities and Nucleic Acid Contents by Administration of Testosterone

다국어 초록 (Multilingual Abstract)

To observe some metabolic effects of the testosterone injection in different dietary condition, femele albino rats from a pure strain weighing 203g to 271g were used in these experiments. The animals were divided into three diet groups, the standard, the high carbohydrate and the high protein diet group, and these groups were subdivided into seven groups of which two groups (standard and control) were testosterone-noninjected group and the other five were single 0.15ml of testosterone preparation injected ones(equivalent to 15㎎ of testosterone) per rat in according to the periods of the testosterone injection time; the 2-day, 5-day, 10-day, 20-day and 30-day group.
The aninals of the carbohydrate and the high protein diet groups were maintained on each diets for 50 days respectively before the testosterone injection. Since then they were injected a single dosage testosterone intramuscularly. The animals were maintained on each diets and sacrificed 2 to 30 days thereafter.
The alkaline phosphatase actvities in the serum and the liver, and the contents of RNA and DHA in the liver were measured in each period.
The results obtained are summarized as follows:
1. Alkaline phosphatase activities in the serum and the liver were significantly decreased in comparison with the standard diet group by feeding with the high carbohydrate diet for 50 days.
2. Alkaline phosphatase activities in the liver were significantly decreased in comparison with the standard diet group by feeding with the high protein diet for 50 days, but the activities in the serum showed no marked changes under the same dietary condition.
3. RNA contents in the liver were slightly decreased in comparison with the standard diet group by feeding with the high carbohydrate diet or the high protein diet for 50 days.
4.DNA contents in the liver were slightly increased in comparison with the standard diet group by feeding with the high carbohydrate diet for 50 days, but the contents in the liver were slightly decreasd by feeding with protein diet for 50 days.
5. Serum alkaline phosphatase activities in the standard diet group before testosterone injection were decreased in comparison with the control group on 2-day after the testosterone injection, and showed no marked changes on 5-day to 20-day while the activities were decreased on 30-day.
6. Serum alkaline phosphatase activities in the high carbohydrate and the high protein diet group were significantly increased in comparison with the control group on 5-daly after testosterone injection.
7. Liver alkaline phosphatase activities in the standard diet group were decreased in comparison with the control group at each period after the testosterone injection but the high carbohydrate diet group showed a tendency to increase at each period, and the high protein diet group showed marked increase on 5-day and 20-day.
8. Liver RNA contents in the standard diet group were slightly decreased in comparison with the control group on 2-day but no remarkable change on 30-day after the testosterone injection, and those in the high carbohydrate and the high protein diet groups were slightly increased on 5-day.
9. Liver DNA contents in the standard and the high protein diet group were slightly decreased in comparison with the control group during each period after testosterone injection but those in the high carbohyate diet group showed no marked changes in each period.

목차 (Table of Contents)

• Ⅰ. 緖論
• Ⅱ. 實驗材料 및 方法
• A. 食餌
• B. 實驗動物
• C. Testosterone 投與 및 試料採取
• D. 測定方法
• Ⅲ. 實驗成績
• A. 血淸과 肝臟中 alkaline phosphatase 活性
• B. 肝臟中 RNA 및 DNA 含量
• Ⅳ. 總括 및 考察
• Ⅴ. 結論
• References