상황버섯, 발아현미, 발아현미 상황버섯균사체의 IgE 생합성에 대한 면역조절에 관한 비교연구 = Comparative study about immunity modulation on IgE biosynthesis of Phellinus linteus (PL), Germinated Brown Rice (BR) and Phellinus linteus Grown on Germinated Brown Rice (PB)

다국어 초록 (Multilingual Abstract)

Background and objectives: To compare the immunomodulatory effects on the amount of IgE synthesis, the distribution of T-cells and the amount of cytokine secretion in the MLN (Mesenteric Lymph Node) between Phellinus linteus (PL), Germinated Brown Rice (BR) and Phellinus linteus Grown on Germinated Brown Rice (PB) and thereby to examine their relationships with atopic dermatitis.
Materials and methods: All the extracts of PL, BR and PB were dissolved in the propylene glycol. Then, male mice aged 4 weeks were divided into four experimental groups, each of which comprised ten mice. These mice were provided with experimental foods in a certain environment and then orally given each extract. Through a retro-orbital bleeding, the peripheral blood was sampled. This blood sample was used for an analysis of IgE.
Results: The concentration of serum IgE was significantly decreased in the experimental groups where PL, BR or PB were administered as compared with the control group (Fig, 1-1). Besides, the concentration of serum IgE was also significantly decreased in the experimental groups where PL, BR or PB were administered in the MLN as compared with the control group (Fig 1-1). The number of CD4+ T-cells was significantly greater in the experimental groups where PL and PB were administered as compared with the control group. But the number of CD8+ T-cells was the smallest in the experimental group where PL was administered(Table 1). Besides, of all the extracts, BR had an effectiveness in enhancing the concentration of IFN-γ (Interferon-γ) to the greatest extent in the MLN (Fig, 1-2).
Conclusions: It has been reported that Th2 cytokines and serum IgE are relatively higher in more than 80% of patients with atopic dermatitis. Our results showed that PB reduced the amount of the secretion of Th2 cytokine. Thus, by modulating the amount of IgE synthesis, it had an immunosuppressive effect on various types of inflammatory markers. It is also presumed that PB might reduce the amount of the secretion of pro-inflammatory cytokines in MLN. It can therefore be inferred that PB might also have a treatment effect on atopic dermatitis. Further studies are warranted to examine the relationship between PB and atopic dermatitis.

국문 초록 (Abstract)

상황버섯, 발아현미, 발아현미 상황버섯균사체가 각각 생쥐의 MLN(Mesenteric Lymph Node) 림프구 내에서 형성되는 IgE의 생성량, T-세포 분포 및 cytokine 분비량 등에 미치는 면역조절 효과를 서로 비교하므로 아토피피부염과의 관련성 여부를 알아보았다. 데이터는 모두 평균±SEM으로 나타내었고 각 실험 그룹의 평균 차는 던칸의 다중범위검정(Duncan's multiple range test)과 함께 실시된 일원 공분산분석(one-way ANOVA)으로 그 값을 구하였다. 이들 분석을 위해 SPSS 10.0(SPSS institute, Chicago, IL)을 사용하였다. 그 결과, 혈청 IgE 농도는 PL, BR, 또는 PB를 투여한 실험군이 대조군에 비해 유의하게 감소한 것으로 나타났다(Fig, 3-1). 또한 모든 추출물 특히 BR은 MLN 림프구 내에서 IFN-γ농도를 크게 높이는 효과가 있었으며(Fig, 3-2), 모든 추출물은 Th1(IFN-γ 제외) cytokine 및 Th2 cytokine의 분비량을 크게 감소시키는 효과가 있었다(Fig, 3-2). 아토피피부염 환자의 80% 이상에서 Th2 우세와 혈청 IgE가 증가하는 것으로 보고되고 있다. 따라서 아토피피부염에서도 그 효과를 볼 수 있을 것으로 사료되며 향후 아토피피부염과의 관련성 여부에 대한 더 많은 연구가 이루어져야 할 것이다.

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