염분변화가 넙치의 혈액학적 성상에 미치는 영향
저자
발행사항
부산 : 동의대학교, 2001
학위논문사항
학위논문(박사)-- 동의대학교 대학원 : 생물학과 2001년 12월
발행연도
2001
작성언어
한국어
KDC
529.34 판사항(4)
발행국(도시)
부산
형태사항
xi, 96장 : 삽도 ; 26cm
일반주기명
참고문헌 : 89-96장
소장기관
The effects of sudden changes of salinity on the stress response (cortisol, glucose, Na+, K+, Cl-, total protein, ALT, AST, hematocrit, RBC, hemoglobin, MCV, MCHC and MCH) and physiological change (survival, growth, feed effect and condition factor) of the cultured olive flounder of large (FL) and small (FS) size were examined in a flow through seawater (SW) culture system with 6 tanks (water vol. 270ℓ/tank).
The experiment was performed in duplicate, water exchange rate was 32 times water volume and dissolved oxygen was maintained over 5 ppm. The experimental fish were sampled from different tanks before and after increased water temperature, after 4 days and at the end of the experiment. Blood samples were obtained from the caudal blood vessel complex using heparinized syringes within 1 min non-anesthesia. Hematological factors were analysed using auto blood analyzer at once. The samples were kept in vacutainer treated with sodium fluoride potassium oxalate and common tubes (1.5 cc) on ice for less than 5 min before centrifugation. Blood plasma was then collected and stored in freezer at -70℃ until analysis. Plasma cortisol concentration was measured by radioimmunoassay. Glucose, Na+, K+, Cl-, total protein, ALT and AST were analysed using a chemical system. The growth of the fish was recorded on days 0, 15 and 30.
The salinity was decreased from SW (35‰) to freshwater (0‰, FW) within 30 minutes and maintained at FW for 24 hours (acute hypo-salinity stress). Flounder of large and small size were tested in a flow through SW culture system with 35‰ and 15‰ SW tanks for 30 days (chronic hypo-salinity stress). The salinity was decreased (0‰) and increased (35‰) directly from rearing fish in 15‰ SW for 30 days (after rearing in chronic hypo-salinity). The hypo-salinity transportation was performed for 6 hours in square boxes by mixture experimental groups of water temperature (15℃ and 20℃) and anesthesia (50 ppm/ℓ) at SW (15‰).
The salinity was increased directly from seawater to FW and remained for 24 hours (density difference to acute hypo-salinity change).
In acute hypo-salinity stress, the levels of blood hematocrit at 3 hours (SW) in FW was significantly increased from 16.1±1.3% to 23.4±4.1%. At 6, 12, 24 hours were higher than the first time (18.0±1.5~24.6±3.7%). Plasma cortisol level at 1 hour (SW) in FW was significantly increased from 1.6±0.2 ng/㎖ to 22.8±3.8 ng/㎖, at 3 and 24 hours increased 9.3±1.8 ng/㎖ and 9.5±0.2 ng/㎖, respectively. The survival in FW was decreased at 72 hours (92.0±5.7%), 96 hours (50±2.8%), 120 hours (20±11.2%) and 144 hours (0%).
In chronic hypo-salinity stress, growth of body weight at 15 days in the control group (83.2 g) was higher than that in group (76.9 g). Control and experimental groups at 30 days showed 106 g and 89.2 g, respectively. In flounder of small size, Control and experimental groups at 30 days showed 21.1 g and 23.0 g, respectively. In flounder of large size, feed conversion rate showed significant differences between control (42.4±2.0%) and experimental (37.8±4.3%) groups. In flounder of small size, condition factors showed significant differences between control (10.5±0.2) and experimental (11.2±0.4) groups.
After chronic hypo-salinity stress, plasma cortisol level at the beginning of the experiment (4.4±1.9 ng/㎖) showed no significant differences at 3 (3.7±1.9 ng/㎖) and 24 hours (2.8±1.1 ng/㎖) in 35‰ group. But, in 0‰ group at 24 hours increased 47.9±18.6 ng/㎖. In Na+ level (0‰ group) at 24 hours decreased from 166.8±10.7 mEq/ℓ to 149.0±9.4 mEq/ℓ.
In hypo-salinity transportation, plasma cortisol level before transportation was 2.4±0.1 ng/㎖. After transportation the levels showed no significant differences in CWT+35‰ group (16.7±12.8 ng/㎖), but, all groups increase of it according to transportation. The levels of plasma K+ after transportation in NWT+15‰ group showed no significant differences (4.5±1.1 mEq/ℓ), but, all groups displayed an increase of it according to transportation.
In density difference to acute hypo-salinity change, the levels of plasma cortisol at 3 hours in the control group was significantly increased from 2.4±0.3 ng/㎖ to 20.4±14.7 ng/㎖, and at 24 hours increased 66.9±18.0 ng/㎖. In the experimental group at 3 and 24 hours it increased 45.9±36.0 ng/㎖ and 264.1±141.5 ng/㎖, respectively. In the case of plasma glucose in the control group it increased from 29.4±29.8 mg/㎗ to 121.6±35.5 mg/㎗, but at 24 hours decreased 29.4±16.2 mg/㎗. In the experimental group at 3 and 24 hours it increased 230.8±48.5 mg/㎗ and 133.5±54.2 mg/㎗, respectively.
In density difference, Plasma cortisol and glucose levels showed no significant differences during the experiment period in all experimental groups. Growth of total length at 30 days in 30, 60 and 90 fish groups showed 20.1±2.8%, 19.3±1.0% and 18.6±1.0%, respectively.
After density difference stress, plasma glucose level at 1 hour in 30 fish group increased from 21.0±4.0 g/㎗ to 42.1±15.6 mg/㎗. In 60 fish groups it increased from 22.1±5.5 mg/㎗ to 35.1±3.2 mg/㎗. Total protein level at 1 hour in 60 fish groups increased from 24.0±2.9 mg/㎖ to 33.8±3.0 mg/㎖, at 24 hours it was 29.8±2.2 mg/㎖.
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