콘택트렌즈 재질 및 침착 단백질에 따른 균 흡착 정도와 다목적용액의 살균력 비교 = Comparisons of Adherence Level of Micro-organisms According to Contact Lens Materials and Protein Deposition and Disinfection Efficacy of Multipurpose Solution

다국어 초록 (Multilingual Abstract)

Purpose: The present study was aimed to compare the difference in adherence level of microorganisms accordingto contact lens materials and protein deposition and to evaluate disinfection efficacy of multipurpose solution.
Methods: The evaluations of micro-organisms' adherence and disinfection efficacy of multi-purpose solutionwere conducted by employing the Part 2. Regimen Procedure for Disinfecting Regiments in the DisinfectionEfficacy Testing under the “FDA Evaluation Criteria & Method”. Results: Pseudomonas aeruginosa, Serratiamarcescens, Candida albicans except Staphylococcus aureus adhered more on etafilcon A lens and disinfectionefficacy of total 4 products investigated was almost perfect except Candida albicans. The 3 micro-organismsexcept Serratia marcescens adhered more to albumin-predeposited lens. Disinfection efficacy of multi-purposesolution was higher against the micro-organisms adhered to albumin-deposited lens than against the microorganismsadhered to the lysozyme-deposited lens. Furthermore, disinfection efficacy of multi-purpose solutionwas different according to types of micro-organisms. Conclusions: It was revealed that the type of microorganisms,the lens materials and type of absorbed tear protein affected the amount of adhered micro-organismsto contact lens and that adhesion of tear protein could induce the change of disinfection efficacy of multi-purposesolution. It suggest that the hygienic condition of contact lens can vary by these factors influencing ondisinfection efficacy and the occurrence of adverse effect can be affected.

국문 초록 (Abstract)

목적: 콘택트렌즈 재질 및 렌즈에 침착된 단백질 종류에 따른 균의 흡착력을 비교하고 관리용품의 살균력 차이를측정하고자 하였다. 방법: FDA 평가기준 및 방법인 Disinfection Efficacy Testing의 Part 2. Regimen Procedure ForDisinfecting Regimens에 따라 균 흡착 및 관리용품의 살균력 측정실험을 실시하였다. 결과: 포도상구균을 제외한 녹농균, 세라티아균, 칸디다균은 etafilcon A 렌즈에 더 많이 흡착되었으며, 4종의 다목적용액은 칸디다균을 제외한 모든 균에서 거의 완벽한 살균력을 나타냈다. 눈물 단백질을 침착시킨 렌즈에서 세라티아를 제외한 모든 균은 알부민침착 렌즈에서의 흡착이 많았으며, 다목적용액의 살균력은 라이소자임 침착 렌즈에 흡착된 균보다 알부민 침착 렌즈에 흡착된 균에서 높은 것으로 나타났다. 또한, 균의 종류에 따라 다목적 용액의 살균력이 달랐다. 결론: 균의 종류, 콘택트렌즈 재질, 침착된 눈물 단백질의 종류가 콘택트렌즈에 흡착되는 균의 양에 영향을 주며, 눈물 단백질의침착은 다목적용액에 의한 살균력에 변화를 초래할 수 있음을 밝혔다. 이러한 살균력에 영향을 주는 요인에 의해콘택트렌즈 위생상태가 달라지며 부작용 발생 여부가 영향을 받을 수 있음을 제안한다.

참고문헌 (Reference)

• 1 고은경, "콘택트렌즈용 다목적용액(MPS)의 세포증식저해 및실험용 토끼 각막에 미치는 영향" 한국현미경학회 36 (36): 141-153, 2006
• 2 Cheng L, "Wettability of siliconehydrogel contact lenses in the presence of tear-film components" 28 (28): 93-108, 2004
• 3 Hambleton P, "The sensitivity of gram-negative bacteria, recovered from aerosols, to lysozyme and other hydrolytic enzymes" 61 (61): 197-204, 1970
• 4 Chang KY, "Studies on the struture and function of lysozyme : The effect of pH and cation concentration on lysozyme activity" 229 (229): 496-503, 1971
• 5 Giraldez MJ, "Soft contact lens surface profile by atomic force microscopy" 87 (87): 475-481, 2010
• 6 Borazjani RN, "Relative primary adhesion of Pseudomonas aeruginosa, Serratia marcescens and Staphylococcus aureus to HEMA-type contact lenses and an extended wear silicone hydrogel contact lens of high oxygen permeability" 27 (27): 3-8, 2004
• 7 Lowry OH, "Protein measurement with the Folin phenol reagent" 193 (193): 265-275, 1951
• 8 Michaud L, "Overwear of contact lenses : increased severity of clinical signs as a function of protein adsorption" 79 (79): 184-192, 2002
• 9 Osserman EF, "Lysozyme" Academic Press 169-186, 1974
• 10 Subbaraman LN, "Influence of protein deposition on bacterial adhesion to contact lenses" 88 (88): 959-966, 2011
• 11 Zhang S, "In vitro deposition of lysozyme on etafilcon A and balafilcon A hydrogel contact lenses : Effects on adhesion and survival of Pseudomonas aeruginosa and Staphylococcus aureus" 28 (28): 113-119, 2005
• 12 Food and Drug Administration, "Guidance for Industry-Premarket notification(510(k)) guidance document for contact lens care products"
• 13 Imamura Y, "Fusarium and Candida albicans biofilms on soft contact lenses : model development, influence of lens type, and susceptibility to lens care solutions" 52 (52): 171-182, 2008
• 14 Kravchenko NA, "Effect of salt and other factors on lysozyme activity" 32 (32): 618-623, 1967
• 15 Tran VB, "Dynamics of flagellum and pilus mediated association of Pseudomonas aeruginosa with contact lens surfaces" 77 (77): 3644-3652, 2011
• 16 Brennan NA, "Deposits and symptomatology with soft contact lens wear" 27 (27): 75-100, 2000
• 17 Giraldez MJ, "Contact lens hydrophobicity and roughness effects on bacterial adhesion" 87 (87): 426-431, 2010
• 18 Nathan E, "Contact lens complications" Elsevier Korea 236-249, 2008
• 19 Ma KJ, "Contact lens" Daehakseolim 82-322, 1995
• 20 Berman ER, "Biochemistry of the eye" Springer 79-80, 1991
• 21 Bruinsma, GM, "Bacterial adhesion to surface hydrophilic and hydrophobic contact lenses" 22 (22): 3217-3224, 2001