Ginsenosdie F1과 EGCG의 상승작용에 의한 자외선조사에 의한 세포 사멸 방지 = Combining Ginsenoside F1 with (-)-Epigallocatechin Gallate Synergistically Protects Human HaCaT Keratinocytes from Ultraviolet B-Induced Apoptosis

다국어 초록 (Multilingual Abstract)

Ginsenosides and green tea extracts show a variety of biomedical efficacies such as anti-aging, anti-oxidation and anti-tumor-promotion effects. (-)-Epigallocatechin-3-gallate (EGCG) has been reported to inhibit the UVB-induced apoptosis by increasing the Bcl-2-to-Bax ratio. We have previously shown that ginsenoside F1 protects human HaCaT cells from ultraviolet-B (UVB)-induced apoptosis by maintaining constant levels of Bcl-2 and Brn-3a. Here, we investigate the combined effect of ginsenoside F1 and EGCG on the protection of human HaCaT keratinocyte against UVB-induced apoptosis. When treated individually, although 5 μM ginsenoside F1 and 50 μM EGCG protected cells from UVB-induced apoptosis, 2 μM ginsenoside F1 or 10 μM EGCG treatment showed very little protection effect. However, cotreatement of 2 μM ginsenoside F1 and 10 μM EGCG successfully protected HaCaT cells from UVB-induced cell death. As expected, combining ginsenoside F1 and EGCG efficiently prevented UVB-induced decrease of Bcl-2 and Brn-3a expression. In addition, cotreatment with ginsenoside F1 and EGCG prevented the dephosphorylation of Rb, whereas individual treatment with ginsenoside F1 or EGCG failed to prevent the dephosphorylation of Rb even at high concentrations.