SSR 분자마커를 이용한 색소옥수수 계통들에 대한 계통유연관계 및 집단구조 분석

국문 초록 (Abstract)

본 연구는 총 50개의 SSR primer를 이용하여 색소옥수수(색소종실 옥수수 12계통, 색소찰 옥수수 12계통) 및 비색소옥수수(종실옥수수 2계통, 찰옥수수 5계통) 계통들에 대하여 유전적 다양성, 계통유연관계 및 집단구조를 분석하였다.1. 그 결과 50 개의 SSR primer들은 색소 및 비색소옥수수 31계통들에서 총 282개의 대립단편을 증폭시켰으며, SSR primer들에서 증폭된 대립단편의 수는 최소 2개에서부터 최대 11개까지의 범위로 나타나 평균 5.64개가 증폭되었다. MAF(major allele frequency)는 0.23(bnlg279)에서 0.90(umc2338)의 범위로 나타나, SSR primer 당 평균 0.45개로 나타났고, 유전적 다양성(GD)값은 0.17(umc2338)에서 0.86(bnlg279)의 범위로 나타나, SSR primer 당 평균 0.67의 값을 나타내었다. 2. 31개의 색소 및 비색소옥수수 계통들의 집단구조를 분석한 결과, 이들 옥수수 계통들은 Groups I, II, III, IV, V, admixed로 구분되었다. Group I은 찰옥수수 1계통, 색소종실옥수수 1계통, 색소찰옥수수 5계통이 포함되었고, Group II는 찰옥수수 1계통, 색소찰옥수수 3계통이, Group III는 색소종실옥수수 3계통, Group IV는 색소종실옥수수 2계통이, Group V는 종실옥수수 2계통, 찰옥수수 2계통, 색소종실옥수수 5계통이 포함되어 있었다. 그리고 admixed group에는 찰옥수수 1계통, 색소종실옥수수 1계통, 색소찰옥수수 4계통 이 포함되어 있었다.3. UPGMA법에 의한 계통유연관계 분석 결과, 31개의 색소 및 비색소옥수수 계통들은 유전적 유사성 27.4%의 수준에서 크게 3개의 그룹으로 나누어졌다. Group I은 10개의 옥수수 계통(종실옥수수 2계통, 찰옥수수 2계통, 색소종실옥수수 6계통)을 포함하고 있었고, Group II는 20개의 옥수수 계통(찰옥수수 3계통, 색소종실옥수수 5계통, 색소찰옥수수 12계통)을, 그리고 Group III은 색소종실옥수수 1계통을 포함하고 있었다. 따라서 본 연구의 결과는 앞으로 강원도 농업기술원 옥수수시험장에서 색소옥수수 자식계통들에 대한 선발과 품종 육성 연구에 유용한 정보를 제공할 것으로 생각된다.

다국어 초록 (Multilingual Abstract)

In this study, genetic diversity, genetic relationships, and population structure were investigated among colored maize lines (12 colored normal maize lines and 12 colored waxy maize lines) and non-colored maize lines (2 normal maize inbred lines and 5 waxy maize inbred lines) using 50 SSR markers. A total of 282 alleles were identified at all the loci with an average of 4.84 and a range between 2 and 11 alleles per locus. The major allele frequency varied from 0.23 to 0.90 with an average of 0.45. The genetic diversity values varied from 0.17 to 0.86 with an average of 0.67. To evaluate the population structure, STRUCTURE 2.2 program was employed to confirm genetic structure. The 31 colored and non-colored maize lines were separated with based on the membership probability threshold 0.8, and divided into groups I, II, III, IV, V, and admixed group. The cluster tree generated using the described SSR markers recognized three major groups at 27.4% genetic similarity. Group I included 10 maize lines (2 normal maize lines, 2 waxy maize lines, 6 colored normal maize lines), and Group II included 20 maize lines (3 waxy maize lines, 5 colored normal maize lines, 12 colored waxy maize lines). Group III consist of only 1 colored normal maize inbred line. The present study has demonstrated the utility of SSR analysis for the study of genetic diversity, genetic relationships, and the population structure among colored and non-colored maize lines.

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