A Cervus Elaphus and Eucommia Ulmoides Extract Blend Attenuates Muscle Atrophy by Regulating Protein Metabolism and Antioxidant Activity

다국어 초록 (Multilingual Abstract)

Here, we investigated whether a mixture of Cervus elaphus and Eucommia ulmoides (1:3, KGC01CE) couldsuppress muscle atrophy in H2O2-induced C2C12 cells and dexamethasone-injected mice. Our results revealed that KGC01CEeffectively safeguarded against H2O2-induced muscle atrophy in C2C12 cells compared with the same mixture at other ratios.
We demonstrated that dexamethasone elicited oxidative stress in muscle tissue and decreased the grip strength and crosssectionalareas of muscle fibers; however, oral administration of KGC01CE (1:3) suppressed these dexamethasone-inducedchanges. Furthermore, oral KGC01CE (1:3) administration suppressed the expression of protein degradation-associated factors,myostatin, muscle RING finger 1, and atrogin-1 in muscle tissue from dexamethasone-injected mice. Oral KGC01CE administrationstimulated protein synthesis by stimulating the PI3K/Akt/mTOR pathway in muscle tissue from dexamethasone-injectedmice. These findings indicate that KGC01CE can suppress dexamethasone injection-induced muscle atrophy by inhibiting proteindegradation and promoting muscle hypertrophy. Therefore, KGC01CE supplementation helps prevent muscle atrophy inmice and may be beneficial against various components of muscle atrophy.