잣나무 수피 추출물중의 플라보노이드에 대하여 = On the Flavonoids of Bark Extractives from Pinus koraiensis

다국어 초록 (Multilingual Abstract)

The Flavonoids of the bark Ectractives from Pinus koraiensis grown in Mt. Jiri were analysed. in this study. Dihydroquercetin(DHQ) was isolated from analysed diethyl ether soluble fraction in the EtOH(95%) extractives from the bark of Pinus koraiensis.
1) Diethyl ether soluble fraction content in the EtOH(95) extractive from the bark of Pinus koraiensis was 14.0%.
2) Development Standard of dihydroquercetin, H and I group among fraction of liquid chromatography were separated a section Rf value 0.4 by TLC.
3) The dried sample was tested by UV spectroscopy. Maximum peak of DHQ in methanol removed 289 nm to 292 nm in methanol mixed with 1N NaOH solution. It was identified to be phenolic compound having aromatic ring. Typical peak of monomer appeared at 1600 and 1800 cm.
4) R values in phenolic compound and hydroxyl appeared 3436 to 3200 cm and aromatic R value was at 1476 cm. It was identified to be phenolic compound having aromatic ring. Typical peak of monomer appeared at 1600 and 1800 cm.
5) The typical hydrogen bonding signal of DHQ by Proton NMR showed at 11.70 ppm between the OH-5 and 4-C=O. The H-6 and the H-8 on the A ring showed at 6.01 and 5.94 ppm, to be double doublet.
Each signal due to the three aromatic protons of the B ring appeared at 7.54 and 7.42 ppm to doublet H-5' : at 7.25 and 7.12 ppm to be doublet H-6' : at 6.96 and 6.86 ppm to be double doublet H-2' on the NMR Spectrum. Downfield removed to meta position between the H-2' and H-6'.