가토 압력과부하 심근비후에서의 Nuclear DNA 의 변화 = Changes of Nuclear DNA in Pressureoverload Hypertrophied Heart of Rabbit

다국어 초록 (Multilingual Abstract)

Background: During the development of cardiac hypertrophy, cytoplasmic contents of cardiac myocytes usually increase and nuclei of myocytes change as well. We used flow cytometry to study the changes of nuclear DNA in cardiac hypertrophy which was experimentally produced by aortic banding in New Zealand White Rabbits. This study was aimed to see how early these nuclear changes can occur and whether these changes correlate to the degree of cardiac hypertrophy. Methods: Each two rabbits were sacrificed at 0-,4-,8-,12- hours, and 1-,2-,4-,5-, and 9- days after experimental aortic constriction and their hearts were studied with flow cytometric analysis. Nuclear DNA was analyzed with FACScan (Becton-Dickinson Co.) after tissue preparatian using modified Hedley and Vindelov method, and staining with Krishan staining buffer. Results: The heart weight to body weight ratio (g/kg) increased progressively as a results of time lapsing after aortic banding. In control hearts (Group I ), flow cytometric analysie showed normal Diploidy pattern in all tissues. In hypertrophied hearts, Proliferative Indicies (S+GM Phase) were increased progressively in Group II (4, 8, 12 hours after aortic banding) and Group III (24 hours to 9 days after aortic banding). An Aneuploidy pattern was noticed in a severely hypertrophied heart produced 9 days aortic banding. Conclusions: Changes of nuclear DNA developed even in the early times in the experimental model of pressureoverload cardiac hypertrophy of rabbit after aortic banding and it was roughly correlated with the degree of heart hypertrophy. Abnormal DNA ploidy pattern can be observed in the severely hypertrophied heart.