生長調節劑 處理가 땅콩의 Callus 形成 및 器官 分化에 미치는 影響 = Effects of Growth Regulators on Callus Formation and Organogenesis in Peanut(Arachis Hypogaea L.) Tissue Culture

다국어 초록 (Multilingual Abstract)

This study was carried out to find out effects of NAA(α-naphthalene acetic acid), 2,4-D (2,4-dichlorophenoxy acetic acid), BA(6-benzyl amino purine) and kinetin(6-furfuryl aminopurine) on callus induction, shoot differentiation and organogenesis from callus of peanut(Arachis hypogaea L.).
The peanut varieties cultured on Murashige and Skoog(MS) medium supplemented with growth regulators were Yeongho, Daekwang and Sinpung.
In shoot tip culture, the effects of growth regulator differed in growth response and numbers of expanded leaves were promoted on MS media containing BA and the shoot and internode elongation were inhibited on MS media containing BA. However, the expanded leaves, shoot and internode elongation and root differentiation were inhibited on MS medium containing NAA. The callus formation was promoted on MS media containing high concentrations of 2,4-D. The shoot growth was inhibited and callus formation was hastened on MS media containing kinetin.
In hypocotyl culture, the callus formation was promoted on MS media containing BA and MS medium supplemented with 5.0㎎/ℓ of NAA and 0.5∼1.0㎎/ℓ of BA was most effective for callus formation, root differentiation was irregular and shoot formation was not observed.
The growth responses of growth regulators differed in organs of varieties ; Sinpung cultivar was sensitive to expansion of leaf and Daekwang and Sinpung cultivars were sensitive to differentiation of root.
The callus formation was originated in the phloem parenchyma of outer region of vascular bundle.