Mouse수정란의 초급속동결에 있어서 Vitrification Solution 개발에 관한 연구 : Ⅱ. Vitrification Solution 내의 비투과성 물질(Ficoll, sucrose)과 평형시간이 초급속동결· 융해후 mouse morulae의 생존율에 미치는 영향 = ###############################################################################################################################################################################################################################################################

다국어 초록 (Multilingual Abstract)

This study was carried out to study effects of the addition level of acetamide and non-permea-ble cryoprotectants(Ficoll, sucrose) in VS(20% glycerol + 10% ethyleneglycol) and equilibration time on the survival of vitrified mouse morulae.
The results are summarized as follows:
1. When 10, 15 and 20% of acetamide were added to the new vitrification solution(20G 10E), FDA-scores of embryos were 4.4(control), 4.4(10%), 3.6(15, 20%), respectively. The addition of acetamide did not affect the survival of forzen-thawed morulae.
2. The survival between 5 min(3.5) and 10 min(4.6), 10 min(4.6) and 20 min(3.2) of equili-bration in 10% sucmse, and 20 min(3.2) and 5 min(4.0), or 10 min(4.3) in 20% sucrose was sig-nificantly different(P<0.05). The highest survival(4.6) was obtained in mouse morulae equilibrat-ed in VS(2OG10E) containing 10% sucrose for 10 minutes.
3. FDA-score of morulae frozen in the new vitrification solution containing 0, 10, 20 and 30% Ficoll was 4.5, 4.2, 4.4 and 4.6, respectively and had no significant effect among concentrations of Ficoll.
The development rate after culture(24h) was 89%(20% Ficoll) and 93% (30% Ficoll), respectively.

목차 (Table of Contents)

• Summary = 17
• Ⅰ. 서론 = 18
• Ⅱ. 재료 및 방법 = 18
• Ⅲ. 결과 및 고찰 = 20
• Ⅳ. 적요 = 23
• Ⅵ. 인용문헌 = 24