RANKL에 의해 유도되는 뼈파괴세포(Osteoclast) 분화에 미치는 모과의 효과 = Effect of Water Extract of Papaya on RANKL-induced Osteoclast Differentiation

국문 초록 (Abstract)

뼈을 흡수하는 뼈파괴세포와 뼈를 형성하는 뼈모세포 사
이의 조화는 뼈 항상성에 중요하다. 특히, 뼈파괴세포 형성
과 활성의 증가는 뼈엉성증, 류마티스 관절염, 치주염과 같
은 뼈 질환을 야기한다. 식물의 천연물질은 새로운 치료제
개발을 위해 많은 관심을 받고 있다. 본 연구의 목적은 뼈파
괴세포의 분화를 억제하는 천연물질의 연구이다. Receptor
activator of nuclear factor-κB ligand (RANKL)에 의해서 포
식세포에서 뼈파괴세포로의 분화는 모과 물 추출물의 농도
증감에 따라 억제되었다. 그러나 모과 물 추출물은 대조군
과 비교했을 때 세포독성은 검출되지 않았다. 모과 물 추출
물은 RANKL에 의해 유도되는 p38과 JNK의 인산화를 억
제하였다. RANKL에 의해 유도되는 c-Fos, NFATc1, TRAP,
OSCAR mRNA는 모과 물 추출물에 의해 억제되었으며 또
한, c-Fos와 NFATc1 단백질의 발현도 모과 물 추출물에 의
해 억제되었다. 이들의 결과로 모과가 뼈 질환 치료에 유용
한 치료제로 이용될 수 있을 것이라 생각된다.

다국어 초록 (Multilingual Abstract)

Balance between bone-resorbing osteoclats and bone-forming osteoblasts is important in bone homeostasis.
In particular, increased osteoclast formation and activity are responsible for bone diseases such as osteoporosis,
rheumatoid arthritis, periodontal disease. Natural metabolites of plants have recently received much attention as lead
compounds for the development of novel therapeutic strategy. The purpose of this study was to search the natural products
to inhibition osteoclast differentiation. Water extract of papaya significantly inhibited receptor activator of nuclear
factor-κB ligand (RANKL)-induced osteoclast differentiation in bone marrow macrophages (BMMs) in a dose dependent
manner. However, water extract of papaya did not affect cytotoxicity when compared with control. Water extract
of papaya inhibited the phosphorylation of p38 and JNK induced by RANKL. The mRNA expression of c-Fos,
NFATcl, TRAP and OSCAR induced by RANKL was inhibited by water extract of papaya treatment. Also, water
extract of papaya suppressed the protein expression of c-Fos and NFATc1 in BMMs treated with RANKL. Taken
together, these results suggest that papaya may be a useful drug in the treatment of bone-related disease.

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