며느리배꼽 잎 유래 캘러스의 부정근 형성에 미치는 지베렐린의 작용

국문 초록 (Abstract)

본 연구는 항산화 기능이 뛰어나다고 알려진 며느리배꼽(Persicaria perfoliata)의 캘러스를 사용하여, 어떤 식물호르몬이 부정근 형성에 영향을 미치는지 알아보기 위한 실험이다. 며느리배꼽의 잎으로부터 캘러스를 유도하고 이로부터 부정근 형성을 관찰하였다. 캘러스 유도의 최적 조건을 구한 결과 1% sucrose, 4.5 μM 2.4-D, 1/2 MS였다. 어떤 식물호르몬이 이 식물의 캘러스에서 부정근 형성 효과를 가지고 있는지 알아보기 위하여 GA3, IAA, 2iP, 2,4-D를 캘러스 조직에 각각 첨가한 결과, GA3와 IAA를 첨가한 배지에서만 부정근이 나타났음을 확인 할 수 있었다. 이를 자세히 조사하기 위해서 GA3와 IAA의 농도별(0.1, 1, 10 mg/l) 부정근 형성, 길이 및 직경을 알아본 결과, 높은 수준의 GA3 또는 낮은 수준의 IAA처리에서 더 많은 부정근 형성을 보였다. 이 두 호르몬 중 어떤 것이 더욱 더 중요한 역할을 하는지 알아보기 위하여 옥신 유입 저해제인 NPA와 지베렐린 생합성 저해제인 PBZ를 처리한 실험을 한 결과, GA3가 IAA 보다 부정근 형성에 더욱 더 중요한 역할이라는 것을 알았다. 하여 지베렐린의 수준을 증가 또는 감소시킨다고 알려진 식물호르몬(IAA, 2iP, kinetin, ABA)들을 GA3와 혼용 처리한 결과, 이 역시 지베렐린이 부정근을 형성하는데 중요한 역할을 한다는 것을 재확인 하였다. 본 연구는 지베렐린이 며느리배꼽의 캘러스에서 부정근 형성을 증진시킨다는 것을 처음으로 밝힌다.

다국어 초록 (Multilingual Abstract)

This study was carried out to investigate the action of phytohormones which influence the adventitious root formation of calli originating from the leaves of Persicaria perfoliata. The optimal medium condition for callus formation was ½-strength MS, 1% sucrose, and 4.5 μM 2,4-D. In order to determine which phytohormones had an effect on the adventitious root formation, the calluses were cultured in various media with different kinds of phytohormones. As a result, the medium with GA3 or IAA was shown to induce root formation. To deeply investigate the effects of GA3 and IAA, calli were cultured in 0.1, 1, and 10 mg/l levels of phytohormones. Numbers of roots formed per callus were 10.9, 14.2, 22.6 in GA3, 5.8, 3.9, 1.1 in IAA, respectively. Therefore, the higher GA3 or the lower IAA concentration, the more roots formed. To confirm this role of GA3 we tested with inhibitors PBZ and NPA. GA3 with PBZ resulted in reduction by 52.4~69.4% compared to GA3 alone. In contrast, GA3 with NPA resulted in an increase by -8~45.6% compared to GA3 alone in root formation. Also, results were determined on the effect of GA3 with other phytohormones on root formation. Kinetin, 2iP and ABA with GA3 had a negative effect, but IAA with GA3 showed a similar result to GA3 alone. From these results we infer GA plays a key role and auxin has subsidiary activity on adventitious root formation. This is the first report that indicates GA3 promotes adventitious root formation from calli in P. perfoliata.

목차 (Table of Contents)

• 서론
• 재료 및 방법
• 결과
• 고찰
• References
• 초록

참고문헌 (Reference)

• 1 양윤정, "국내 자생 자원식물들의 항산화 활성 검색" 한국자원식물학회 24 (24): 1-9, 2011
• 2 Sun, T. P., "The molecular mechanism and evolution of the GA–GID1–DELLA signaling module in plants" 21 : 338-345, 2011
• 3 Li, F., "The effect of 2, 4-D and kinetin on dedifferentiation of petiole cells in Arabidopsis thaliana" 56 : 121-125, 2012
• 4 Kende, H., "The Five"Classical"Plant Hormones" 9 : 1197-1210, 1997
• 5 Aloni, R., "Role of auxin in regulating Arabidopsis flower development" 223 : 315-328, 2006
• 6 Taiz, L., "Plant Physiology" Sinauer Associates 523-593, 2013
• 7 Wilmoth, J. C., "NPH4/ARF7 and ARF19 promote leaf expansion and auxin-induced lateral root formation" 43 : 118-130, 2005
• 8 Kaneko, M., "Loss-of-function mutations of the rice GAMYB gene impair α-amylase expression in aleurone and flower development" 16 : 33-44, 2004
• 9 Seo, M., "Interaction of light and hormone signals in germinating seeds" 69 : 463-472, 2009
• 10 Brian, P. W., "Inhibition of rooting of cuttings by gibberellic acid : with one figure in the text" 24 : 407-419, 1960
• 11 Richards, D. E., "How gibberellin regulates plant growth and development : a molecular genetic analysis of gibberellin signaling" 52 : 67-88, 2001
• 12 Mauriat, M., "Gibberellins inhibit adventitious rooting in hybrid aspen and Arabidopsis by affecting auxin transport" 78 : 372-384, 2014
• 13 Riov, J., "Ethylene and auxin-ethylene interaction in adventitious root formation in mung bean(Vigna radiata)cuttings" 8 : 131-141, 1989
• 14 Caboni, E., "Biochemical aspects of almond microcuttings related to in vitro rooting ability" 39 : 91-97, 1997
• 15 Mattsson, J., "Auxin signaling in Arabidopsis leaf vascular development" 131 : 1327-1339, 2003
• 16 Fu, X., "Auxin promotes Arabidopsis root growth by modulating gibberellin response" 421 : 740-743, 2003
• 17 Pamfil, D., "Auxin control in the formation of adventitious roots" 39 : 307-316, 2011
• 18 Overvoorde, P., "Auxin Control of Root Development" 2 : a001537-, 2010
• 19 Xu, M., "A PIN1 family gene, OsPIN1, involved in auxin-dependent adventitious root emergence and tillering in rice" 46 : 1674-1681, 2005