생사(生絲) 품위향상(品位向上)을 위(爲)한 기초조사(基礎調査) 연구(硏究): 견층(繭層) Sericin과 용체(龍體) 단백질(蛋白質)의 용해거동(溶解擧動)에 관(關)한 연구(硏究)(3) = A Study on Basic Investigation for Quality Improvement of Raw-Silk: A Study on the Dissolving Behavior of Sericin in the Cocoon Shell and Pupa Protein (Bombyx mori L.) (Part 3)

다국어 초록 (Multilingual Abstract)

This study is to investigate the content of crude protein intruded in the sericin of cocoon shell and pupa by treatment of buffer solution (pH 1 to pH 13) 20 ml per 1 gram for 30 and 60 minutes at 30℃, 60℃ and 100±2℃, respectively. The results obtained are summarized as follows. 1. The quantity of total crude protein obtained from cocoon shell and pupa by treatment during 30 minutes at 30℃ was dissolved as the largest quantity of 11.874 mg/g at pH 1 and l5.93mg/g at pH 13, but dissolved the smallest quantity 1.75g/g at pH 5 as known. and tile quantity of crude protein treated for 60 minutes is 13.437mg/g at pH 1 and 22.50mg/g at pH 13. Also, the smallest quantity is 2. 813mg/g at pH 5 as known. 2. By the treatment during 30 minutes at 60℃, the dissolved largest quantity was 13.12mg/g at pH 1 and 21.875 mg/g at pH 13, but the smallest quantity is 2.375mg/g at pH 5 as known. After treatment for 60 minutes at 60℃, the dissolved largest quantity was 17.500 mg/g at pH 1 and 31.56mg/g at pH 13, but the smallest quantity is 3.849 mg/g at pH 5. 3. The dissolved crude protein from the cocoon shell and pupa by treatment for 30 minutes at 100±2℃ was the largest quantity of 147.000mg/g at pH 1 and 398. 125mg/g at pH 13, but the smallest quantity is 75.00mg/g at pH 5 as known. After treatment for 60 minutes at 100±2℃, the largest quantity was 253.76 mg/g at pH 1 and 460.625mg/g at pH 13, but the smallest quantity is 139.375mg/g at pH 5 as known. 4. The dissolved crude protein from the cocoon shell and pupa was not different in quantity by treatment at 30℃ or 60℃. But dissolved crude protein was large quantity from cocoon shell more than pupa, as known. 5. The treatment of cocoon shell and pupa during 60 minutes at 100±20℃ was increased to the dissolved largest quantity of crude protein of 19.20％ at pH 1 and 22.18％ at pH 13 from the cocoon shell and 6.12％ at pH, an d 23.87％ at pH 13 from the pupa. But dissolved crude protein was increased to the larger quantity from pupa more than cocoon shell.