四鹽化炭素 反覆投與 家兎에 對한 NH₄Cl負荷試驗 = Ammonium Chloride Tolerance Tests in Rabbits Intravenousely Administered with Multiple Doses of Carbon Tetrachloride.

다국어 초록 (Multilingual Abstract)

This study was undertaken to evaluate changes in blood levels of ammonia nitrogen (NG₃-N), CO₂ contents and urea nitrogen(BUN) following NH₄Cl administration on toxic hepatitis of rabbits induced by carbon tetrachloride (CCl₄). Fourty rabbits were devided into 4 groups.
Group Ⅰ: One ㎖ of 30％ CCl₄ in olive oil was injected subcutaneously 5 times at intervals of 2 days until the 12th experimental day in 14 animals. Two animals thus treated were sacrificed respectively on the 3rd, 6th, 9th, 12th, 15th, 22nd and 29th day. In addition 2 nontreated animals were sacrificed for control. Blood samples were drawn from each of 14 animals prior to CCl₄ injection for the determination of blood NH₃-N, glutamic pyruvic transaminase (GPT), BUN and serum iron.
Group Ⅱ: Eight animals were similarly treated with CCl₄ and blood samples were drawn every 3rd day until 15th day, and again on 22nd and 29th for determination of the same.
Group Ⅲ: Ammonium chloride tolerance tests were carried out in 8 normal animals and blood samples were drawn for determination of blood levels of NH₃-N, CO₂ contents and BUN before, and at 1/2, 1 1/2, and 2 1/2 hrs. following NH₄Cl administration.
Group Ⅳ: Ammonium chloride tloerance tests were done in 8 animals administered with CCl₄, and blood samples were drawn for the same as in group Ⅲ.
Results were as follows:
1. Active central necrosis of the hepatic lobules was observed only until the 12th experimental day. This necrotic area was almost completely replaced by regenerated hepatic cells in the 22nd day and repair was complete on the 29th day.
2. Normal blood levels of NH₃-N, GPT and serum iron were respectively 77.4±17.5㎍％, 36.7±7.9 Sigma-Frankel units and 130.3±33.6㎍％.
3. Average blood levels of NH₃-N, GPT and serum iron which were analyzed in 8 animals on every 3rd day after CCl₄ injection, were respectively 147.4㎍％, 259.6 Sigma-Frankel units, 18.9㎎％ and 295.8㎍％. After the last CCl₄ injection blood levels of NH₃-N and BUN returned normal at 10 days, serum iron at 17 days but GPT levels were slightly higher than normal even at 17 days.
4. The NH₄Cl tolerance tests conducted in 8 normal animals showed the average blood levels of NH₃-N, CO₂ contents and BUN as follows: prior to, 1/2, 1 1/2, 2 1/2 hr. after NH₄Cl administration. Blood NH₃-N: 70.5, 143, 81, 73.8㎍％; CO₂ contents: 41.4, 38.1, 37.8, 36.8 vol％; BUN: 22.5, 23.6, 25.2, 27.1㎎％.
5. Average blood levels of NH₃-N, CO₂ contents and BUN in 8 animals following NH₄Cl administration on every 3rd day after CCl₄ injection were respectively as follows: prior to, 1/2, 1 1/2, 2 1/2 hr. after NH₄Cl. Blood NH₃-N: 126.6, 203, 137.6, 116㎍％; CO₂ contents: 42.7, 38.4, 38.3, 35.7 vol％; BUN: 19.6, 20.9, 20.8, 21.1㎎％.
Blood ammonia levels were most significantly elevated at 1/2hr. after NH₄Cl loading in both CCl₄ treated and control groups, and elevation rates were 60％ and 100％, respectively. However, elevated amount showed 76.4㎍％ and 72.5㎍％. BUN levels were highest at 2 1/2hr. in both groups and elevation rates were 8％ and 22％. Also elevation amount was 2.6㎎％ and 4.6㎎％. CO₂ contents were most decreased at 2 1/2hr. in both groups and decrease rates were 16％ and 12％, respectively.

목차 (Table of Contents)

• Ⅰ. 緖論
• Ⅱ. 實驗材料 및 方法
• A. 實驗材料
• B. 實驗動物
• C. 組織學的 및 血液化學的 檢査方法
• Ⅲ. 實驗成績
• A. 第1群, CCl₄反覆投與한 肝의 組織學的 變化
• B. 第2群, CCl₄反覆投與時의 血液化學的 檢査
• C. 第3群, 正常對照의 NH₄Cl 負荷試驗
• D. 第4群, CCl₄ 反覆投與時의 NH₄Cl 負荷試驗
• E. 各群의 血中 ammonia의 比較
• F. 第1群과 第2群의 比較
• G. 第3群과 第4群의 NH₄Cl 負荷試驗成績 比較
• Ⅳ. 考按
• Ⅴ. 結論
• 參考文獻