광학현미경 관찰을 위한 동물 조직표본 제작과정에서 고정 및 염색의 중요성

다국어 초록 (Multilingual Abstract)

Microscopy goes through various steps toward identifying the structure and function of tissues,
especially after tissue extraction. In the process of microscopy, the‘fixation’and‘staining’are
indispensable steps. The fixation serves to prevent destruction of morphology through protein
denaturation and promote mordancy of staining. A variety of staining methods can be used. The H&E
staining is one of the methods that were used in microscopy to enhance contrast using hematoxylin
and eosin. The present experiment was carried out to determine the need for tissue fixation and
double staining. Kidney, liver, pancreas and testis of rats were targeted to observe of the tissue
specimens. The results were as follows; the fixed tissue sections were effectively stained and clearly
observed. While the morphology of tissue sections that were not fixed also resulted in much
destruction of the tissues. In conclusion, fixation with formalin and double staining with hematoxylin
and eosin made detailed morphology.