Staphylococcus epidermidis의 성상과 Plasmid profile의 상관에 대해서 = Relationship Between Antibiotics Resistance and Plasmids of Coagulase Negative Staphylococci

국문 초록 (Abstract)

안결막 및 그 외 병소로부터 분리한 23주의 coagulase 음성 포도구균을 분류하고 항생제에 대한 감수성검사와 각균주의 plasmid분포의 검사를 통하여 EtBr처리에 의한 약제내성의 cure와 plasmid소실과의 상관성을 관찰하였다.
penicillin G와 cephalothin에 대해서는 10%내외, ampicillin, noboviocin, erythromycin, tobramycin, gentamycin, kanamycin 및 amikacin에 대해서는 20~30%, 그리고, tetracycline 및 chloramphenicol에 대해서는 각 47.8%와 56.3%의 균주가 내성을 나타내었다.
공시균주 중 17주(73.9%)가 plasmid를 보유하고 있었고 tetrcycline내성주(다제내성주 포함) 11주는 2.9 Mdal의 plasmid를 가진 6주와 이보다 작은 plasmid를 가진 4주로 나누어졌으며, 후자의 4주 중에서는 tetracycline cured strains만 얻을 수 있었고 이들 Tccured strain들 중에는 plasmid소실이 수반되는 균주도 있었다. 2.9Mdal의 plasmid를 가지는 S_16주에서 얻은 cured strain들에서는 tetracycline cured strain들만 plasmid band가 소실되었다.

다국어 초록 (Multilingual Abstract)

Coagulase negative Staphylococci isolated from conjunctiva and other sources were classified and observed the relationship between antibiotics resistance patterns and plasmids profiles of these isolates, also antibiotics curing experiments were carried out with ethidium bromide in accompanying with the loss of plasmids.
Most isolates belonged to Staphylococcus epidermidis(69.6%) and cephalothin was the most sensitive antibiotics(8.7%) next to penicillin, and chloramphenicol was the most resistant drug(56.5%) next to tetracycline.
Seventeen strains harbored plasmids, and eleven tetracycline resistant strains divided into two groups by molecular size of plasmids, i.e. 6 strains of 2.9Mdal and 4 strains of less than this size. From 4 strains of latter group, only tetracycline cured strains were isolated with or without loss of plasmid, however, from one strain of former group, tetracycline, chloramphenicol and erythromycin cured strains in single or in combination with were isolated but only tetracycline cured strains were accompanied with the loss of plasmids compared to parent strain.