신생백서의 저산소 허혈 뇌손상에서 Heme oxygenase억제제의 효과 = Effect of the Heme Oxygenase Inhibitor on the Hypoxic Ischemic Brain Injury in the Neonatal Rat

다국어 초록 (Multilingual Abstract)

Background: The heme oxygenase system catalyzes the conversion of heme to free iron, carbon monoxide and bilirubin. This study was purposed to evaluate the effect of the heme oxygenase inhibitor, Tin protoporphyrin IX (SnPPIX) on the hypoxic ischemic brain injury in a neonatal rat.
Methods: Seven-day old Sprague-Dawley rat pups were used. The rats were divided into two groups; control group (n = 9) and SnPPIX group (n = 6). SnPPIX 50μmol/kg and the dissolvent were administered respectively intraperitoneally. For hypoxic ischemic brain injury, the right common carotid artery was ligated with 5-0 silk and the rats were put in the moisturized hypoxic gas chamber for 150 minutes. Lipid/N-acetyl aspartate and Lipid/Creatine ratio of 1H magnetic resonance spectroscopy were evaluated on the 1st day and the 7th day after hypoxic ischemic brain injury. All rats were sacrificed 2 weeks after hypoxic ischemic brain injury for morphological study.
Results: There were no statistically significant differences between two groups in the result of MRS and Lip/Cr and Lip/NAA ratio on 1th day and 7th day after hypoxic ischemic brain injury. In addition, there was no significant difference in the gross morphological evaluation on the 14th day.
Conclusions: Our results suggest that the pretreatment of the Tin protoporphyrin IX does not affect the degree of brain damage which is caused by apoptosis in the model of hypoxic ischemic brain injury in a neonatal rat. (Korean J Anesthesiol 2006; 50: 706～13)

국문 초록 (Abstract)

본 실험에서 사용한 HO의 억제제는 SnPPIX 로서 0.1N NaOH와 95% ethanol에 용해시키고 0.9% NaCl을 첨가해 pH를 7.2 7.4가 되게 하였다.12) SnPPIX군(n = 9)에 이 용액 50μmol/kg을 저산소 허혈 뇌손상 유발 30분전에 복강으로 투여하였다. 대조군(n = 6)에는 SnPPIX이 첨가되지 않은 용매만을 저산소 허혈 뇌손상 유발 30분전에 복강으로 투여하였다. 저산소 허혈 손상후 생존한 백서들은 제 1일째와 제 7일째에 1H MRS 소견을 얻었으며 저산소 허혈 손상 2주 후에 실험대상 모두에 대해 조직학적 검사를 위한 뇌적출을 시행하였다. SnPPIX이 광감작 되는 것을 피하기 위해 SnPPIX의 처리는 빛을 차단한 상태에서 이루어졌다.1H MRS의 관찰 신생백서들의 마취는 2 mg/kg의 ketamine을 복강 내에 주사하여 실시하였고, Bruker Biospec 4.7T MRI/MRS system (Bruker, Fallanden, Switzerland)을 사용하여 수소자기공명 분광을 얻었다. 본 실험실에서 자체 제작한 원형표면 코일(직경 0.7 cm)을 사용하였으며 double spine echo를 통한 체적선택기법을 이용하여 3 3 4 mm3의 signal voxel을 우측 두정측두부 부위에 선택하여, TR = 3,000 msec, TE = 30 msec, NS = 128로 spine echo signal을 얻었다(Fig. 1). Chemical shift selective sequence로 H2O신호를 억제하였다.

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