양성 계면활성 소독제인 A제품(Dodecyl-di(aminoethyl)-glycine HCI, 10%수용액)과 B제품(Monolauryl-di(aminoethyl)-glycine HCI, 10%수용액)간의 살균력 비교 = Bactericidal activity comparison of amphoteric surfactants, product A(dodecyl-di(aminoethyl)-glycine HCI, 10% water solution) and product B(monolauryl-di(aminoethyl)-glycine HCI, 10% water solution)

국문 초록 (Abstract)

본 병원에서 원내 감염의 원인 균주로 문제시 되는 6균종 18균주에 대해 양성계면활성제인 A제품(dodecyl-di(aminoethyl)-glycine)과 B제품(monolauryl-di(aminoethyl)-glycine)을 가지고 살균력 비교실험을 실시, 다음과 같은 결과를 얻었다.
1. A제품의 경우 0.05%농도에서 30초 접촉한 후 배양한 결과 S. aureus를 제외한 모든 균주에서 colony 형성이 관찰되지 않았다.
2. 충분한 살균효과를 얻기 위해서는 A제품의 경우 0.1%농도에서 2분, B제품은 0.1%에서 5분, 0.5%농도에서 2분이상의 접촉시간이 요구되었다.
3. 전체적인 살균력에 있어 A 92.6%, B 75.9%로 A가 B보다 우수한 효과를 나타냈다.

다국어 초록 (Multilingual Abstract)

To prevent hospital-acquired infections, it is important to sterilize, disinfect completely and choose an appropriate disinfectants. We examined two same kinds of amphoteric surfactants, product A(dodecyl-di(aminoethyl)-glycine HCI,10％ water solution) and product B (monolauryl)-di(aminoethyl)-glycine HCI, 10％ water solution), to compare whether they have equal sterilizing power to same strains.
We used 6 species 18 strains of Staphylococcus aureus, Pseudomonas aeruginosa, E. coli, Klebsiella pneumoniae, Coagulase negative staphylococcus(CNS), and Candida albicans. The organisms diluted with phosphate buffer(1-5×10^(6)/mL), and contacted them with three different concentrations (0.05％, 0.1％, 0.5％) for 0.5, 1, 2, 5mins followed by inoculating them in BHI agar. They were incubated at 38℃ for 24 or 48 hrs depending on the strain type.
As a results, product A and product B sterilized S. aureus within 30 sec at 0.5％ and after 2 mins at 0.5％ respectively. Product A sterilized P. aeruginosa, K pneumoniae, CNS within 30 sec at 0.05％ and product B sterilized them after one minute at 0.05％ concentration.
We found that they had difference in effective concentration and working time. Product a must be used more than for 2 minutes at 0.1％ and product B used more than 0.5％ for 2 minutes.