호중구 기능의 노화에 따른 감소와 간편한 유세포분석법 고안 = Age-Related Decrease and a Simple Flow Cytometric Assay of Neutrophil Function

다국어 초록 (Multilingual Abstract)

Background : We intended to confirm a decline in neutrophil function with aging and to devise a simple neutrophil function test protocol for use in a clinical setting. Reversely, the reliability of this protocol was to be verified by detectability of a decline in neutrophil function with aging. Methods : Whole blood samples from young (thirties, N=32) and old (sixties, N=32) healthy subjects were incubated with the 7-aminoactinomycin D stained Escherichia coli. The mixture was stained by dihydrorhodamine 123 as an oxidative probe. Two kinds of fluorescence were measured by flow cytometry. Results : Phagocytosis was declined with aging as indicated by a decrease in the percentage (form 28.2±9.5% to 21.9±10.9%, P<0.05) and the mean fluorescence intensity (MFI) ratio (from 1.67±0.27 to 1.51±0.27, P<0.05). Oxidative burst had a trend toward a decrease with aging, but the differences were not significant (percentage: from 35.3±13.2% to 32.1±14.1%, P=0.36; MFI ratio: from 5.26±3.23 to 5.08±3.55, P=0.84). Conclusions : The devised protocol in this study could detect a significant decline in neutrophil function with aging, and this protocol may be useful for the assessment of neutrophil function in a clinical setting

국문 초록 (Abstract)

서론 : 본 연구에서는 노화에 따른 호중구 기능의 감소를 평가하고, 이를 위하여 실제 검사실에서 간편히 사용할 수 있는 호중구검사 술식을 고안하였으며, 이 술식의 신뢰성을 노화에 따른 호중구 기능의 감소를 검출할 수 있는지 여부로 검증하고자 하였다.방법 : 30대 건강한 32명의 젊은이군과 60대 건강한 32명의 노인군을 대상으로, 자극으로서 7-aminoactinomycin D 염색Es-cherichia coli를 사용하여 피검자의 전혈과 섞어 항온 후, oxida-tive probe로서 dihydrorhodamine 123을 사용하여 반응시킨 후,두 형광을 유세포분석법으로 측정하였다. 결과 : 포식작용은 노화에 따라 백분율(28.2± 9.5%에서 21.9±10.9%로, P<0.05)과 mean fluorescence intensity (MFI) ratio(1.67± 0.27%에서 1.51± 0.27%로, P<0.05) 분석 모두에서 유의하게 감소하였다. Oxidative burst는 노화에 따라 감소하는 경향이 있었으나, 그 차이가 유의하지 않았다(백분율: 35.3± 13.2%에서 32.1± 14.1%로, P=0.34; MFI ratio: 5.26 ± 3.23에서 5.08±3.55로, P=0.84).
결론 : 본 연구에서 고안한 술식으로 호중구 포식작용의 노화에 따른 유의한 감소를 검출할 수 있었고, 본 술식은 임상 검사실에서 환자를 대상으로 호중구 기능을 평가하는데 유용할 것으로 생각된다.

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