식이로 유도된 고콜레스테롤혈증 흰쥐에서 두유 및 유청 첨가 비숙성 치즈의 보충섭취가 지질 및 항산화 체계에 미치는 영향 = Effects of soymilk and whey protein fortified unripened cheese supplementation on Lipid and antioxidant status in hypercholesterolemia SD rats

다국어 초록 (Multilingual Abstract)

This study are performed to examine the effects of whey protein and soymilk fortified unripened cheese supplementation on lipid metabolism and antioxidant status in male SD rats fed a high cholesterol diet. 32 male SD rats were rando㎖y divided into 4 groups; normal control diet group (NC), Hypercholesterolemic control, (HC), and high fat diet with 5% soymilk (Soymilk)and whey protein (WPC) fortified unripened cheeses groups. Rats were induced hypercholesterolemia by feeding a high cholesterol diet(0.5% cholesterol and 0.2% Sodium cholate) for 4 weeks. Plasma total cholesterol (TC) significantly increased in the HC, Soymilk and WPC compared to the NC. All cheeses supplementation were reduced triglyceride (TG) and AST (alanine aminotransferase) level compared to the HC. Accumulation of hepatic TG was apparent in the HF group, while these effects were improved by supplement with Soymilk and WPC. However hepatic TC level was lower than in WPC compare to the HC group. When the proportion of feces total lipids was calculated, there was a significant increase in the Soymilk and WPC group compared to the HC. Feces TC concentration of WPC was higher than HC but Soymilk level was no significant. In the plasma total antioxidant activity, there was significantly influenced in Soymilk and WPC but not significant difference between the NC and HC. Concentrations of retinol and α-tocopherol were apparent reduced in the HC group, while there were improved by supplement with WPC. Tail DNA, tail moment and tail length for the 200 μM H2O2 induced DNA damage analyze were increased in HC compared with the NC. On the other hand, soymilk and whey protein fortified unripened cheeses have significant inhibition on the DNA damage. These results showed that the soymilk and whey protein fortified unripened cheese feeding decreased the lipid profiles in plasma and inhibited accumulation of the hepatic total lipid, TG and TC, as well as stimulated fecal extraction. Therefore, soymilk and whey protein fortified unripened cheese exerts significant hypocholesterolemic benefits through the influence of antioxidant status.