培養條件에 따른 食中毒 細菌의 發育에 關한 硏究 = Studies on the Growth of Food-borne Pathogens According to the Cultural Condition

국문 초록 (Abstract)

食中毒 原因菌에 대한 培養條件에 따른 發育狀態를 調査하고자 食水, 牛乳, 豚肉에 E. coli, Sal. typhimurium, Staph. aureus, Y. enterocolitica, Lac casei를 一定量 接種한 후 4℃, 20℃ , 37℃에 3주간 保管하면서 菌의 發育, 增殖狀態를 觀察하였고, 低溫에서도 增殖이 可能한 Y. enterocolitica와 다른 菌株들을 牛乳에 混合培養하여 역시 4℃, 20℃, 37℃에 保管하면서 菌의 發育·增殖 狀態를 관찰한 결과 다음과 같은 결론을 얻었다.
1) 食水에 있어서 保管溫度에 관계없이 Y. enterocolitica만이 활발하게 증가하였으며, E. coli, Sal. typhimurium, Staph. aureus, Lac casei 등은 현저하게 감소하였다.
2) 牛乳와 豚肉에 있어서 4℃ 보관시에는 Y. enterocolitica만이 增加하였으며, 20℃와 37℃에 보관시에는 모든 菌株들이 增加하였다.
3) 牛乳에 Y. enterocolitica와 다른 菌株들을 混合培養時 4℃로 保管했을 때 Y. enterocolitica는 增加하였으나, 다른 菌株들은 감소하였다. 20℃에서는 Y. enterocolitica가 다른 菌株들에 비해 優勢한 增加 傾向을 보였으나, 37℃에서는 다른 菌株들이 Y. enterocolitica보다 優勢하였다. 특히 Lac. casei는 보다 더 우세하였다.

다국어 초록 (Multilingual Abstract)

In order to investigate the growth of the food-borne pathogens according to the storage temperature, the growth and multiplication of E. coli, Sal. typhimurium, Staph. aureus, Y. enterocolitica and Lac. casei in the potable water, milk, and meat were tested during the storage for 3 weeks at 4℃, 20℃ and 37℃ after inoculation of constant concentration of the tested bacteria. And the competitive growth and multiplication of Y. enterocolitica which is able to grow well at low temperature and the other pathogens in milk were tested during the storage at 4℃, 20℃ and 37℃.
The result were as follows:
1. In the potable water, only Y. enterocolitica was grown well without temperature, but E. coli, Sal. typhimurium, Staph. aureus, and Lac. casei were reduced remarkably.
2. In the milk and the meat, only Y. enterocolitica was increased during the storage at 4℃. All bacteria tested were increased noticeably during the storage at 20℃ and 37℃.
3. In the mixed incubation of Y. enterocolitica and the other bacteria in milk, Y. enterocolitica was increased during the stage at 4℃ and the others were reduced. At 20℃, Y. enterocolitica was increased more noticeably than the other bacteria. At 37℃ the other bacteria, especially Lac. casei, were increased more noticeably than Y. enterocolitica.