레시틴이 강화된 인삼 추출물 제조 방법

국문 초록 (Abstract)

본 연구는 레시틴이 강화된 홍삼 추출물을 제조하는 방법을 개발하기 위하여 수행되었다. 우선 대두유 레시틴을 대두유와 일정 비율로 혼합하여 LCS를 제조하였다. 고형분 함량이 10%인 홍삼 물추출물과 LCS의 비율을 3:1로 하여 혼합하여 vortexing한 뒤 혼합액을 원심분리하여 오일 층과 수용액 층을 분리하였다. 수용액 층에 대하여는 레시틴과 사포닌의 정성 및 정량분석을 행하였다. 홍삼 추출물에 대하여는 LCS처리 전?후 지방산 조성과 조지방 함량도 조사하였다. 또한, LCS를 처리한 홍삼 추출물에 대하여는 농축 후 4℃, 25℃, 40℃/6개월 Falcon 튜브에 넣어 보관하면서 상부 1/3과 하부 1/3의 레시틴 함량을 조사함으로써 안정성을 조사하였다. LCS처리로 인한 홍삼 추출물의 조지방 함량은 변화 없었고, 지방산 조성 변화는 oleic acid의 증가 이외에는 특별한 결과를 관찰할 수 없었다. 홍삼 추출물로의 레시틴 이행율은 대두유에 대한 레시틴의 첨가 비율이 증가함에 따라 정비례적으로 증가하였다. LCS-처리 전?후 홍삼 추출물 중의 지방산 조성과 진세노사이드 함량은 유의한 차이가 없는 것으로 나타났다. TLC 및 HPLC 조사 결과 역시 LCS-처리 전ㆍ후 차이가 없는 것으로 나타났다. 상기 3가지 조건에 6개월간 보관하면서 보관용 튜브의 상ㆍ하부 1/3의 레시틴 함량과 사포닌 조성 변화를 조사한 결과 차이가 없는 것으로 보아 LCS-처리 홍삼 농축액의 안정성은 만족할 만한 것으로 사료된다. 이상의 결과로부터 레시틴 함유 대두유 혼합액으로 홍삼 추출물을 처리하는 방법은 홍삼 추출물에 레시틴을 강화시킬 수 있는 안정성이 뛰어나고 처리 공정이 간편한 방법이라 판단한다.

다국어 초록 (Multilingual Abstract)

This study was carried out to develop the method of preparing lecithin-fortified ginseng extract. Firstly, soybean lecithin was mixed with soybean oil (LCS) in varying ratio (2.5%, 5%, 10% and 20%). Then, one part volume of LCS was mixed with three parts volume of ginseng extract with 10% solid matter content and the mixture was vortexed vigorously. Finally, the mixture was spinned at the speed of 3,000 rpm for 30 minutes to separate oil and aqueous ginseng extract layer (AG). AG was then subjected to qualitative and quantitative analysis of phospholipids and ginsenosides. Fatty acid composition and crude fat content before and after LCS was determined. Stability of lecithin in ginseng extract was determined by analyzing phospholipid content in the one third upper and lower layer of the concentrated AG in Falcon tubes while storing the LCS-treated concentrated AG in 4, 25 and 40℃ for 6 months. Ratio of lecithin transferred to AG increased with the increase in lecithin content of soybean oil. There was no significant change in fatty acid composition and crude fat content, and ginsenoside content in the ginseng extract before and after LCS treatment. TLC and HPLC pattern of saponin fraction before and after treating the ginseng extract with LCS demonstrated no observable difference. There was no change in lecithin content in the upper and lower one third layer of ginseng extract in the tubes after storing the concentrated AG in 4, 25 and 40℃ for 6 months. Ginsenosides HPLC pattern was not changed when stored the LCS-treated ginseng extract in those conditions for six months, indicating satisfiable stability of the LCS-treated concentrated ginseng extract. From these results, it can be concluded that treatment of the ginseng extract with lecithin containing soybean oil is a labor-effective method with satisfiable stability to fortify lecithins to ginseng extract.

목차 (Table of Contents)

• Abstract
• 서론
• 재료 및 방법
• 결과 및 고찰
• 요약
• 감사의 글
• 문헌

참고문헌 (Reference)

• 1 "Unidimensional thin-layer chromatography of phospholipid on boric acid-impregnated plates" 23 : 660-663, 1982
• 2 "The soy revolution." Evans M. and Company, Inc., 43-47, 1999
• 3 "Soy lecithin reduces plasma lipoprotein cholesterol and early atherogenesis in hypercholesterolemic monkeys and hamsters: beyond linoleate." 140 : 147-153, 1998
• 4 "Saponins composition in American ginseng leaf and berry assayed by high-performance liquid chromatography." 54 : 2261-2266,
• 5 "Polyacetylenic compounds, ACAT inhibitors from the roots of Panax ginseng." 53 : 919-922, 2005
• 6 "Official methods of analysis. 17th ed." Association of official analytical chemists, 2000
• 7 "Nutrition for health and health care. 2nd ed." Wadsworth, Thomson Learning, 60-61, 2001
• 8 "Lecithin increases plasma free choline and decreases hepatic steatosis in long-term total parenteral nutrition patients" 1363-102 1370, 1992
• 9 "Health assistant food and biofunctional food. 2nd ed" Hyoil, 172-178, 2000
• 10 "Fat-free foods supplemented with soy stanol-lecithin powder reduce cholesterol absorption and LDL cholesterol" 103 : 577-581, 2003
• 11 "Evidence of memory improvement by phosphatidylcholine supplement at fetus and neonate -Studies of basal forebrain cholinergic neuronal activities-" 32 : 864-869, 1999
• 12 "Effects of Panax ginseng extract on lipid metabolism in humans" 48 : 511-513, 2003
• 13 "Effect of plant stanol tablets on low-density lipoprotein cholesterol lowering in patients on statin drugs" 97 : 376-379, 2006
• 14 "CPMP/QWP/122/02, rev 1; Guideline on stability testing: stability testing of existing active substances and related finished products" 2003
• 15 "Advanced nutrition." Mosby-Year Book 105-106, 1995