정량적 전자토모그래피를 위한 Beer's law의 실험적 검증 = Experimental Examination of the Beer's law for Quantitative Electron Tomography

다국어 초록 (Multilingual Abstract)

This study has examined experimentally the Beer's law which is a precondition for quantitative electron tomography. We used carbon support film and latex spheres, which have similar absorption coefficients with biological samples, as the test samples to take a tilt-series of images for electron tomography. First, the 3D information of carbon film and latex spheres was obtained by electron tomography. Then, the regression analysis on the relationship between the intensities of the incident and the transmitted beams in a tilt series was carried out to examine the Beer's law. The regression results with RMS error of 0.976 show the linear intensity variations of the transmitted beam as the tilt angles were increased. In addition, the relative absorption coefficients of carbon support film and latex spheres calculated experimentally through the Beer's law were 1.71 (5) and 2.67 (6)/${\mu}m$, respectively. The absorption coefficients remained constant within a full tilt range. Therefore, it is expected that quantitative electron tomography could be performed for biological samples by applying Beer's law provided the exact intensity of incident beam can be obtained under the thoroughly controlled experimental conditions.

참고문헌 (Reference)

• 1 김진규, "전자토모그래피의 정량적 분석에서 대물렌즈 조리개의 영향" 한국현미경학회 38 (38): 285-291, 2008
• 2 Pozsgai I, "Thickness determination by measuring in the TEM at 200 kV" 68 : 69-75, 1997
• 3 Levine ZH, "Theory of bright-field scanning transmission electron microscopy for tomography" 97 : 2005
• 4 Hayat MA, "Principles and techniques of electron microscopy-biological applications" Cambridge university press 158-, 2000
• 5 Gilbert P, "Iterative methods for the three-dimensional reconstruction of an object from projections" 36 (36): 105-117, 1972