비색 및 전류법을 이용한 백금-다중벽 탄소 나노 튜브 및 그래핀 산화 나노리본 복합체의 나노자임 활성 연구 = Nanozyme Activity Study of Platinum-Decorated Multi-Walled Carbon Nanotube/Graphene Oxide Nanoribbon Composites Using Colorimetry and Amperometry

다국어 초록 (Multilingual Abstract)

The nanozyme activity of a platinum-incorporated multi-walled carbon nanotube and graphene oxide nanoribbon composite (Pt/MWCNT-GONR) was systematically investigated using colorimetric and chronoamperometric analyses. The nanozyme composite was prepared by inducing nanoribbon formation to enhance MWCNT oxidation via microwave treatment, followed by the loading of platinum particles using a self-reduction approach. The formation of nanoribbons and the attachment of platinum particles on the composite were confirmed by transmission electron microscopy and X-ray diffraction analysis. The peroxidase (POD)-like activity of the synthesized Pt/MWCNT-GONR nanozyme was first evaluated using a colorimetric assay by reacting it with 2 mM 3,3'5,5'-tetramethylbenzidine (TMB) in the presence of 2 mM hydrogen peroxide (H₂O₂). By analyzing the change in absorbance as a function of time in the initial reaction region, the specific activity of the Pt/MWCNT-GONR nanozyme was calculated to be 1.6768 U·mg^-1. In addition, after reacting the Pt/MWCNT-GONR nanozyme under the same concentrations of H₂O₂ and TMB for 3 min, a potential of -0.1 V (vs. Ag/AgCl) corresponding to the reduction potential of TMB was applied, and the POD-like activity was measured by chronoamperometry. In particular, when the concentrations of TMB and H₂O₂ were held constant, increasing the amount of nanozyme enhanced the TMB oxidation reaction, leading to a corresponding increase in the steady-state current change (ΔI). These results confirm the successful synthesis of a POD-like nanozyme.