Adventitious Shoot Regeneration from Petiole and Stem Explants of Pseudolysimachion longifolium (L.) Opiz

다국어 초록 (Multilingual Abstract)

We developed a shoot multiplication protocol for Pseudolysimachion longifolium (L.) Opiz using in vitro cultured seedlings derived from in vitro germinated seeds. To determine the effects of different plant growth regulators (PGRs) on shoot induction and the number of shoots, P. longifolium leaves, petioles, and stems were cultured on half-strength Murashige and Skoog (MS) medium supplemented with 3% sucrose, 0.3% GelriteTM, 0.05 mg L-1 1-naphthaleneacetic acid, and five different types of cytokinins (6-benzylaminopurine, kinetin, thidiazuron (TDZ), zeatin, and 2-isopentenyladenine at 2 mg L-1). Among the five cytokinins tested, stem explants cultured on medium containing TDZ exhibited the best shoot induction (100%) and the highest number of shoots per explant (1.83±0.20). Shoot induction from petiole explants was highest when they were cultured on medium containing zeatin or TDZ. However, no shoot induction was observed from stem or petiole explants when cultured on medium containing kinetin, nor from leaf explants for any of the cytokinin treatments. In vitro generated P. longifolium plantlets were acclimatized in a greenhouse and showed normal growth and development with 90% survival. In conclusion, a regeneration protocol was developed that can be applied for the mass propagation and conservation of P. longifolium.