桃仁承氣湯 및 그 構成藥物이 瘀血病態模型에 미치는 影響 = Study on the Effects of Taorenchengqitang(桃仁承氣湯) and Its Components on Blood Stasis Model

국문 초록 (Abstract)

瘀血은 한의학의 독특한 病理槪念으로서 생리적 功能을 상실한 血液이 體內 일정 部位에 凝聚되어 形成한 일종의 病理的 産物이다. 최근에는 瘀血證의 진단표준에 대한 定量化硏究가 많이 진행되어 임상지단 및 활용성이 더욱 높아지고 있으며, 瘀血의 治法으로는 活血化瘀法을 기본으로 兼證에 따라 기타 治法이 配伍되고 있다. 본 실험에 사용한 桃仁承氣湯은 通下活血法에 해당하는 方劑로서, 《傷寒論》에 처음 수록된 이래 下焦의 瘀熱蓄結의 證候를 치료하는데 사용되어 왔다. 이에 저자는 桃仁承氣湯 및 그 構成 藥物이 瘀血에 충분히 효과가 있을 것으로 판단되어 白鼠의 尾靜脈에 dextran을 注入하여 瘀血病態를 유발시키고 약물의 水煎濃縮液을 經口投與하여, 血小板數, fibrinogen 量, prothrombin time(PT), hematocrit(Ht)値, fibrinogen degradation product(FDP) 濃度, 체표온도를 측정하였으며, 또한 hydrocortisone acetate(HA)로 어혈병태를 유발시켜 血小板數, fibrinogen 量, PT, Ht 値, 全血粘度 및 血漿粘度를 측정하여 두 어혈병태모형에서의 실험결과를 살펴보고 또 이를 서로 비교한 결과 有意한 결과를 얻었다. 특히 적외선체열진단기로 대조군과 실험구의 체표온도 변화를 측정하여 유의성있는 결과를 얻은 것은 하나의 성과라 할 수 있다.

다국어 초록 (Multilingual Abstract)

In order to investigate the effects of Taorenchengqitang and its components on both dextran-induced blood stasis model(Dx Model) and hydrocortisone acetate(HA)-induced blood stasis model(HA Model), rats of Dx Model treated with 10% dextranaqueous solution (i.v. injection, 1.1㎖/200g/day through caudal vein, once) were orally administrated liquid extract of Taorenchengqitang and its components, and then observed platelet, fibrinogen, prothrombin time, hematocrit, FDP level and dermal temperature. Another rats of HA Model (i.m. injection 5㎎/200g/day into the muscular rump, for 7 days) were orally administrated Taorenchengqitang and its components, thereafter measured platelet, fibrinogen, prothrombin time, hematocrit, whole blood viscosity and plasma viscosity.
The results were summarized as follows:
1. Platelet was increased significantly in order of Taorenchengqitang, Rhei Undulati Rhizoma, Sodii Sulfas, Cinnamomi Ramulus on Dx Model and in order of Rhei Undulati Rhizoma, Persicae Semen, Taorenchengqitang, Sodii Sulfas as compared with data of control group on HA Model.
2. Fibrinogen Level was increased significantly in order of Rhei Undulati Rhizoma, Sodii Sulfas, Taorenchengqitang, Persicae semen compared with data of control group both on Dx Model and on HA Model.
3. Prothrombin time was shortened significantly in order of Rhei Undulati Rhizoma, Persicae semen, Taorenchengqitang Sodii Sulfas on Dx Model and in order of Rhel Undulati Rhizoma, Sodii Sulfas, Taorenchengqitang, Cinnamomi Ramulus, Persicae Semen compared with data of control group on HA Model.
4. Hematocit was sightly increased in sample groups compared with data of control group on Dx Model, but was stitistically insignificant.
5. FDP was deceased significantly in order of Rhei Undulati Rhizoma, Persicae Semen, Taorenchengqitang, Glycyrrhizae Radix compared with data of control group on Dx Model.
6. Dermal temperature was increased significantly in order of Persicae Semen ,
Taorenchengqitang after 1hr next to dextran injection; and in order of Persicae Semen, Taorenchengqitang after 2hrs next to dextran injection compared with data of control group on Dx Model.
7. Whole blood viscosity and plasma viscosity were decreased significantly in the most of groups, especially in the low degree of shear rate, compared with data of control group on HA Model. The Cytotoxic effects of Dictamni Cortex Radicis(白蘚皮) and Manitis Squama(穿山甲) on Human cancer cell-lines.