반응표면분석에 의한 대구부산물 열수 추출조건의 최적화 = Optimization of Hot Water Extraction Conditions for Cod Byproduct by Response Surface Methodology Analysis

다국어 초록 (Multilingual Abstract)

To determine optimal conditions for extraction of cod byproduct, Box-Behnken experimental design was applied. Independent factors were extraction temperature(80℃, 98℃, 121℃), extraction time(2h, 3h, 4h) and addition of water(1.3x, 2x, 2.7x). Response variables were extraction yield, crude protein, crude ash and calcium contents. General composition of cod byproduct was moisture(71.74%), crude protein(16.63%), crude fat(0.52%), crude ash(6.86%) and calcium(2.06%). By RSM(response surface methodology) analysis of cod byproduct extraction, extraction temperature greatly affected extraction yield and crude protein content(P<0.01). Crude ash(P<0.01) and calcium(P<0.1) content were largely affected by the addition of water. Optimum extraction condition were extraction temperature of 121℃, extraction time of 2.2h and addition of water of 2.6x. Predicted characteristic values were yield of 8.64%, crude protein content of 86.80mg/g, crude ash content of 3.4mg/g and calcium content of 25.37μg/g at optimal condition.

국문 초록 (Abstract)

대구부산물 열수 추출조건을 최적화하기 위하여 Box-Behnken계획법을 이용하여 추출온도(80, 98, 121℃), 추출시간(2, 3, 4hr) 가수량(1.3, 2, 2.7 배수)을 독립변수로 하고 반응변수로는 수율, 조단백 함량, 조회분 함량, 칼슘 함량을 설정 하였다. 실험 결과는 반응표면분석법을 이용하여 대구부산물 열수 추출조건의 최적화 조건을 결정하였다. 대구부산물의 일반 성분 함량은 수분 71.74%, 조단백 16.63%, 조지방 0.52%, 조회분 6.86%, 칼슘 함량 2.06%였다. 대구부산물의 경우 근육이 혼재 되어 있으므로 순수한 대구뼈 보다는 조단백 및 조지방 함량은 높았고, 조회분 및 칼슘 함량은 낮았다. 추출물 반응변수 특성 값의 반응표면분석 결과 추출온도가 증가함에 따라 수율과 조단백 함량은 유의적으로 증가하였고(P<0.01) 가수량이 증가함에 따라 조회분 함량(P<0.01)과 칼슘 함량(P<0.1) 유의적으로 증가하였다. 그리고 추출 최적조건은 추출온도 121℃, 추출시간 2.2hr, 가수량 2.6배수로 나타났다. 이때 예측 특성값으로 수율8.64%, 조단백 함량 86.80mg/g, 조회분 함량 3.4mg/g, 칼슘 함량 25.37μg/g으로 나타났다.

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