大食細胞의 肥滿細胞 果粒 飽食에 關한 硏究 = Uptake and Degradation of Mast Cell Granules by Macrophages in the Rat

다국어 초록 (Multilingual Abstract)

The present study was concerned with the interaction between macrophages and mast cell granules under the light microscope.
After treatment of compound 48/80 for degranulation of mast cell, mesentery and peritoneal fluid were taken at 30 min, 1hr, 2hr, 4hrs, 8hrs, and 16 hrs. At the same time, the isolated peritoneal cells were studied.
Each preparation containing nacrophages, mast cells and its granules was stained with toluidine blue for metachromasia.
Uptake and degradation of metachromatic granules by the macrophages at each time interval were observed.
In the mesentery, phagocytosis of large metachromatic graules by macrophages were small at 38 min and increased, gradually and significantly, at 8 hrs after injection of compound 48/80. The metachromatic mast cell granules in the macrophage cytoplasm were disintegrated into small dust-like particles and agregated into a few small vacuoles with weakly metachromasia at 16 hours.
In vitro and in vivo experiments with peritoneal cell, the mast cell granules were maximum uptaken by macrophages at 4 hrs after compound 48/80 treatment. The phagocytosed granules and their metachromasia at 8hrs, were shown to be less than those at 4hrs. At 16 hrs, the nacrophages had not large metachromatic mast cell granules but a few small vacuoles including dust-like metachromatic particles were observed. At that time the granules in the macrophage became into particle like deposits, and aggregated into small vacuoles.
Size of the macrophages was not changed, and large vacuoles in the cytoplasm were not observed at the time lapse.
Above resutls suggest that macrophages have a role in the degradation of mast cell granules.