사람의 유방암 세포주와 미성숙 랫드에서 정향피의 비스페놀 A 독성방어 효과 = Protective Effect of the Stem Bark of Syringa velutina on Bisphenol-A in the Human Breast Cancer Cell Line and Immature Rat

다국어 초록 (Multilingual Abstract)

The inhibitory activity against bisphenol-A (BPA), one of well-known endocrine disrupters was examined with the water extracts prepared from the Stem Bark of Syringa velutina (SBS). In this study, we have investigated the effect of SBS on the toxicity caused by BPA in human breast cancer cell line, MCF-7 cells and immature Sprague-Dawley rats. In the estrogen receptor-mediated proliferation assay using MCF-7 cells, BPA (16 ng/ml) induced the cell proliferation, but the water extract of SBS inhibited BPA-induced cell proliferation in a dose-dependent manner. These results are associated with PARP degradation and specific cleavage of anti-apoptotic protein Bcl-2 of apoptotic regulatory factors. Additionally, the BPA (400 mg/100 g) significantly induced the increase of the uterine and virginal weights, while SBS (50 mg/100 g) showed the inhibitory action against BPA, i.e. caused the increase of estrogen-related organ weights in immature rat uterotrophic assay. Taken together, the present data suggest that SBS may have anti-toxicity activities against BPA in vitro and in vivo systems. SBS may be capable of inhibiting adverse effects of BPA such as reproductive disorder.

목차 (Table of Contents)

• ABSTRACT
  
• 서 론
  
• 재료 및 방법
  
• 결 과
  
• 고찰 및 결론
  
• 감사의 글
  
• 참고문헌

참고문헌 (Reference)

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• 2 "^비임상시험기준" 2000식품의약품안정청고시제2000-63호
• 3 "^비임상시험기준" 2000식품의약품안정청고시제2000-63호
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• 8 "Immature uterotrophic assay is more sensitive than ovariectomized uterotrophic assay for the detection of estrogenicity of p-nonylphenol in Sprague-Dawley rats" 109-115, 2000
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• 15 "An assessment of the biological evidence" 109-227, 1998