해양 미생물에 의한 폴리하이드록시알카노에이트 생산의 최근 동향 = Recent Trends in The Production of Polyhydroxyalkanoates Using Marine Microorganisms

국문 초록 (Abstract)

오일 피크, 기후변화 그리고 미세 플라스틱과 관련된 석유합성 플라스틱의 생산과 사용은, 지속 가능한 인류 생활을 위협하는 범세계적 이슈로 대두되고 있다. 이러한 문제를 해결하기 위해 다양한 생분해성 친환경 바이오플라스틱 소재가 대안이 되고 있으며, 그중 주목받고 있는 소재 중 하나는 polyhydroxyalkanoates (PHA) 생분해성 바이오플라스틱이다. 본 총설은 PHA 생산공정에 이용될 수 있는 해양 미생물의 산업적 활용 이점과 현재까지 밝혀진 해양미생물의 생산성을 비교하고, 이들이 산업에서 활용되기 위해 필요한 연구개발 현황에 대해 조사하였다. 조사 결과 해양미생물로부터 생산된 PHA는 석유합성 플라스틱이 보유한 다양한 물리적 특성을 띄는 중합체 소재로의 대체 가능성과, 배지 제조 시 해수를 사용할 수 있는 장점, 특별한 멸균 과정이 필요치 않은 장점, 그리고 분리 정제 과정 등에서 비용 절감의 장점을 제공할 수 있음을 확인하였다. 하지만 해양미생물의 PHA 생산성은 육상에서 분리된 상용화 균주에 비해 다소 떨어지는 효율을 보이는 것을 확인하였다. 이러한 문제점을 해결하기 위해 최근 선진 omics 기반 합성 미생물학 기반 기술을 활용하여, 고밀도 연속배양기술 개발, PHA 효율 증진 및 다양한 시장 요구에 필요한 생분해성 플라스틱 소재 개발이 미래 플라스틱 대체 소재 개발에 필요한 연구임을 확인하였다.

참고문헌 (Reference)

• 1 박대후 ; 김범수, "식물성 오일로부터 Ralstonia eutropha 의 Polyhydroxyalkanoates 합성 특성" 한국생물공학회 25 (25): 239-243, 2010
• 2 이찬우, "미생물 합성에 의한 poly(3-hydroxybutyrate)의 분자량과말단관능기 제어" 한국염색가공학회 30 (30): 130-140, 2018
• 3 Elain, A., "Valorisation of local agro-industrial processing waters as growth media for polyhydroxyalkanoates(PHA)production" 80 : 1-5, 2016
• 4 Bhattacharyya, A., "Utilization of vinasse for production of poly-3-(hydroxybutyrate-cohydroxyvalerate)by Haloferax mediterranei" 2 : 1-10, 2012
• 5 Salgaonkar, B., "Utilization of sugarcane bagasse by Halogeometricum borinquense strain E3for biosynthesis of poly(3-hydroxybutyrate-co-3-hydroxyvalerate)" 4 : 50-, 2017
• 6 Tan, D., "Unsterile and continuous production of polyhydroxybutyrate by Halomonas TD01" 102 : 8130-8136, 2011
• 7 Christensen, M., "Towards highthroughput screening (HTS) of polyhydroxyalkanoate (PHA) production via Fourier transform infrared (FTIR)spectroscopy of Halomonas sp. R5-57 and Pseudomonas sp. MR4-99" 18 : e0282623-, 2023
• 8 Dilkes-Hoffman, L. S., "The rate of biodegradation of PHA bioplastics in the marine environment : a meta-study" 142 : 15-24, 2019
• 9 Defoirdt, T., "The bacterial storage compound poly-β-hydroxybutyrate protects Artemia franciscana from pathogenic Vibrio campbellii" 9 : 445-452, 2007
• 10 Koller, M., "Techniques for tracing PHA-producing organisms and for qualitative and quantitative analysis of intra-and extracellular PHA" 15 : 558-581, 2015
• 11 Hartmann, R., "Tailor-made olefinic medium-chainlength poly [(R)-3-hydroxyalkanoates] by Pseudomonas putida GPo1 : batch versus chemostat production" 93 : 737-746, 2006
• 12 Numata, K., "Synthesis of poly-and oligo (hydroxyalkanoate) s by deepsea bacteria, Colwellia spp., Moritella spp., and Shewanella spp" 45 : 1094-1100, 2013
• 13 Quillaguamán, J., "Synthesis and production of polyhydroxyalkanoates by halophiles : current potential and future prospects" 85 : 1687-1696, 2010
• 14 Moshood, T. D., "Sustainability of biodegradable plastics : new problem or solution to solve the global plastic pollution?" 5 : 100273-, 2022
• 15 Lee, S. Y., "Stimulatory effects of amino acids and oleic acid on poly(3-hydroxybutyric acid)synthesis by recombinant Escherichia coli" 79 : 177-180, 1995
• 16 Tan, G. Y. A., "Start a research on biopolymer polyhydroxyalkanoate(PHA) : a review" 6 : 706-754, 2014
• 17 Eronen-Rasimus, E., "Sea-ice bacteria Halomonas sp. strain 363 and Paracoccus sp. strain 392 produce multiple types of poly-3-hydroxyalkaonoic acid (PHA) storage polymers at low temperature" 87 : e00929-e00921, 2021
• 18 Kshirsagar, P., "Scale up production of polyhydroxyalkanoate(PHA)at different aeration, agitation and controlled dissolved oxygen levels in fermenter using Halomonas campisalis MCM B-1027" 4 : 512-517, 2012
• 19 Lamberti, F. M., "Recycling of bioplastics : routes and benefits" 28 : 2551-2571, 2020
• 20 Sabapathy, P. C., "Recent developments in Polyhydroxyalkanoates(PHAs)production–A review" 306 : 123132-, 2020
• 21 Chiulan, I., "Recent advances in 3D printing of aliphatic polyesters" 5 : 2-, 2017
• 22 Sheu, D. S., "Rapid detection of polyhydroxyalkanoate-accumulating bacteria isolated from the environment by colony PCR" 146 : 2019-2025, 2000
• 23 Solaiman, D. K. Y., "Rapid and specific identification of medium-chain-length polyhydroxyalkanoate synthase gene by polymerase chain reaction" 53 : 690-694, 2000
• 24 Akdoğan, M., "Purification and characterization of polyhydroxyalkanoate (PHA) from a Bacillus megaterium strain using various dehydration techniques:PHA purification from B. megaterium using various dehydration techniques" 93 : 2292-2298, 2018
• 25 Takahashi, R. Y. U., "Prospecting for marine bacteria for polyhydroxyalkanoate production on low-cost substrates" 4 : 60-, 2017
• 26 Kim, B. S., "Production of poly(3-hydroxybutyric acid)by fed-batch culture of Alcaligenes eutrophus with glucose concentration control" 43 : 892-898, 1994
• 27 Son, H. J., "Production of poly (hydroxybutyric-co-hydroxyvaleric) acid by Pseudomonas sp. HJ" 10 : 349-356, 1995
• 28 Mahansaria, R., "Production enhancement and characterization of the polyhydroxyalkanoate produced by Natrinema ajinwuensis(as synonym)≡ Natrinema altunense strain RM-G10" 107 : 1480-1490, 2018
• 29 Danis, O., "Preparation of poly(3-hydroxybutyrate-co-hydroxyvalerate)films from halophilic archaea and their potential use in drug delivery" 19 : 515-524, 2015
• 30 Don, T. M., "Preparation and characterization of poly(hydroxyalkanoate)from the fermentation of Haloferax mediterranei" 17 : 1425-1438, 2006
• 31 Mazhandu, Z. S., "Potential impact of the Covid-19 pandemic on plastic medical waste management in South Africa: a narrative review" IEEE 2021 : 2021
• 32 Hezayen, F. F., "Polymer production by two newly isolated extremely halophilic archaea : application of a novel corrosion-resistant bioreactor" 54 : 319-325, 2000
• 33 Hong, J. W., "Polyhydroxybutyrate production in halophilic marine bacteria Vibrio proteolyticus isolated from the Korean peninsula" 42 : 603-610, 2019
• 34 Crutchik, D., "Polyhydroxyalkanoates(PHAs)production : A feasible economic option for the treatment of sewage sludge in municipal wastewater treatment plants?" 12 : 1118-, 2020
• 35 Riedel, S. L., "Polyhydroxyalkanoates production with Ralstonia eutropha from low quality waste animal fats" 214 : 119-127, 2015
• 36 Alsaadi, A., "Polyhydroxyalkanoate(PHA)biopolymer synthesis by marine bacteria of the malaysian Coral Triangle region and mining for PHA synthase genes" 10 : 2057-, 2022
• 37 Hai, T., "Polyhydroxyalkanoate(PHA)accumulation in sulfate-reducing bacteria and identification of a class III PHA synthase(PhaEC)in Desulfococcus multivorans" 70 : 4440-4448, 2004
• 38 Neoh, S. Z., "Polyhydroxyalkanoate synthase(PhaC) : the key enzyme for biopolyester synthesis" 4 : 87-101, 2022
• 39 Abd El-malek, F., "Polyhydroxyalkanoate nanoparticles produced by marine bacteria cultivated on cost effective Mediterranean algal hydrolysate media" 328 : 95-105, 2021
• 40 Dhangdhariya, J. H., "Polyhydroxyalkanoate from marine Bacillus megaterium using CSMCRI's Dry Sea Mix as a novel growth medium" 76 : 254-261, 2015
• 41 Naser, A. Z., "Poly(lactic acid)(PLA)and polyhydroxyalkanoates(PHAs), green alternatives to petroleum-based plastics : a review" 11 : 17151-17196, 2021
• 42 Zhao, Y. X., "Poly(3-hydroxybutyrate-co-3-hydroxyvalerate)production by haloarchaeon Halogranum amylolyticum" 99 : 7639-7649, 2015
• 43 Wang, F., "Poly(3-hydroxybutyrate)production with high productivity and high polymer content by a fed-batch culture of Alcaligenes latus under nitrogen limitation" 63 : 3703-3706, 1997
• 44 Van-Thuoc, D., "Poly (3-hydroxybutyrate-co-3-hydroxyvalerate)production by a moderate halophile Yangia sp. ND199 using glycerol as a carbon source" 175 : 3120-3132, 2015
• 45 Ronďošová, S., "Optimization of growth conditions to enhance PHA production by Cupriavidus necator" 8 : 451-, 2022
• 46 Mayr, L. M., "Novel trends in highthroughput screening" 9 : 580-588, 2009
• 47 Plackett, D., "Multifunctional and Nanoreinforced Polymers for Food Packaging" Woodhead Publishing Ltd 498-526, 2011
• 48 Anjum, A., "Microbial production of polyhydroxyalkanoates(PHAs)and its copolymers : a review of recent advancements" 89 : 161-174, 2016
• 49 Rai, R., "Medium chain length polyhydroxyalkanoates, promising new biomedical materials for the future" 72 : 29-47, 2011
• 50 Mirjalili, F., "Mechanical and morphological properties of polypropylene/nano α-Al2O3 composites" 2014 : 1-12, 2014
• 51 Shim, H. J., "Mass production of poly(3-hydroxybutyrate)by fed-batch cultures of Ralstonia eutropha with nitrogen and phosphate limitation" 9 : 751-756, 1999
• 52 Ghosh, S., "Macroalgal biomass subcritical hydrolysates for the production of polyhydroxyalkanoate(PHA)by Haloferax mediterranei" 271 : 166-173, 2019
• 53 Linger, J. G., "Lignin valorization through integrated biological funneling and chemical catalysis" 111 : 12013-12018, 2014
• 54 Rodríguez-Contreras, A., "High production of poly(3-hydroxybutyrate)from a wild Bacillus megaterium Bolivian strain" 114 : 1378-1387, 2013
• 55 Kim, S. W., "High production of Poly-β-hydroxybutyrate(PHB)from Methylobacterium organophilum under potassium limitation" 18 : 25-30, 1996
• 56 Lee, S. Y., "High cell density cultivation of Escherichia coli W using sucrose as a carbon source" 15 : 971-974, 1993
• 57 Mathuriya, A. S., "Handbook of Ecomaterials" Springer International Publishing 1-29, 2017
• 58 Mohammadipanah, F., "Halophiles. Sustainable Development and Biodiversity. vol 6" Springer 277-321, 2015
• 59 Tan, D., "Grand challenges for industrializing polyhydroxyalkanoates(PHAs)" 39 : 953-963, 2021
• 60 Crisafi, F., "From organic wastes and hydrocarbons pollutants to polyhydroxyalkanoates : bioconversion by terrestrial and marine bacteria" 14 : 8241-, 2022
• 61 Choi, J., "Factors affecting the economics of polyhydroxyalkanoate production by bacterial fermentation" 51 : 13-21, 1999
• 62 Chen, C. W., "Enzymatic extruded starch as a carbon source for the production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate)by Haloferax mediterranei" 41 : 2289-2296, 2006
• 63 Zheng, Y., "Engineering biosynthesis of polyhydroxyalkanoates(PHA)for diversity and cost reduction" 58 : 82-93, 2020
• 64 Juengert, J., "Determination of polyhydroxybutyrate(PHB)content in Ralstonia eutropha using gas chromatography and nile red staining" 8 : e2748-, 2018
• 65 Mitra, R., "Current developments on polyhydroxyalkanoates synthesis by using halophiles as a promising cell factory" 19 : 1-30, 2020
• 66 Gagnon, K., "Crystallization behavior and its influence on the mechanical properties of a thermoplastic elastomer produced by Pseudomonas oleovorans" 25 : 3723-3728, 1992
• 67 Yadav, B., "Concomitant production of value-added products with polyhydroxyalkanoate(PHA)synthesis : a review" 337 : 125419-, 2021
• 68 Tsuge, T., "Class IV polyhydroxyalkanoate(PHA)synthases and PHA-producing Bacillus" 99 : 6231-6240, 2015
• 69 Marschessault, R. H., "Chiral poly(β-hydroxyalkanoates) : an adaptable helix influenced by the alkane sidechain" 12 : 158-165, 1990
• 70 Surendran, A., "Can polyhydroxyalkanoates be produced efficiently from waste plant and animal oils?" 8 : 169-, 2020
• 71 Singh Saharan, B., "Biotechnological production of polyhydroxyalkanoates : a review on rrends and latest developments" 2014 : 1-18, 2014
• 72 Koller, M., "Biosynthesis of high quality polyhydroxyalkanoate co-and terpolyesters for potential medical application by the archaeon Haloferax mediterranei" 253 : 33-39, 2007
• 73 Lee, E., "Biosynthesis of copolyesters consisting of 3-hydroxybutyric acid and medium-chain-length 3-hydroxyalkanoic acids from 1, 3-butanediol or from 3-hydroxybutyrate by Pseudomonas sp. A33" 42 : 901-909, 1995
• 74 Cervantes-Uc, J. M., "Biosynthesis and characterization of polyhydroxyalkanoates produced by an extreme halophilic bacterium, Halomonas nitroreducens, isolated from hypersaline ponds" 117 : 1056-1065, 2014
• 75 Stanley, A., "Bioplastics for Sustainable Development" Springer 277-294, 2021
• 76 Ray, S., "Biomedical applications of polyhydroxyalkanoates" 57 : 261-269, 2017
• 77 Wei, Y. H., "Biodegradable and biocompatible biomaterial, polyhydroxybutyrate, produced by an indigenous Vibrio sp. BM-1 isolated from marine environment" 9 : 615-624, 2011
• 78 Rahman, M. H., "An overview of non-biodegradable bioplastics" 294 : 126218-, 2021
• 79 Bindu, J., "Advances in Sustainable Polymers. Materials Horizons: From Nature to Nanomaterials" Springer 2019
• 80 Reddy, S. V., "A novel Bacillus sp. accumulating poly (3-hydroxybutyrateco-3-hydroxyvalerate) from a single carbon substrate" 36 : 837-843, 2009
• 81 Koller, M., "A new wave of industrialization of PHA biopolyesters" 9 : 74-, 2022
• 82 Revelo Romo, D. M., "A most effective method for selecting a broad range of short and medium-chainlength polyhidroxyalcanoate producing microorganisms" 10 : 348-357, 2007
• 83 Chen, G. Q., "A microbial polyhydroxyalkanoates(PHA)based bio-and materials industry" 38 : 2434-, 2009
• 84 Choi, J. E., "A lipophilic fluorescent LipidGreen1-based quantification method for high-throughput screening analysis of intracellular poly-3-hydroxybutyrate" 5 : 48-, 2015
• 85 Rajpoot, A. S., "A comprehensive review on bioplastic production from microalgae" 56 : 171-178, 2022