Studies on Mycological Characteristics and Composition Analysis for Breeding Wolfiporia cocos

다국어 초록 (Multilingual Abstract)

This study was carried out to determine the basic mycelial culture conditions for Poria cocos growth. According to colony diameter and mycelial density, suitable media for mycelial growth were Malt yeast extract, Potato dextrose agar, Yeast extract agar, and Yeast malt agar. The optimum temperature for mycelial growth was between 25 and 35oC, and the optimum pH value was between 4 and 7. Carbon and nitrogen sources were fructose and yeast extract. The optimum C/N ratio was about 10 to 1 with 2% glucose. Other minor components for optimal growth were thiamine-HCl and nicotinamide as vitamins, acetic and lactic acid as organic acids, and MgSO4·7H2O and FeSO4·7H2O as mineral salts. Wolfiporia cocos is a well-known traditional medicine in China, Japan, Korea, and other Asian countries owing to its numerous therapeutic properties. With the aim to determine the morphology and genetic characteristics of W. cocosten strains of W. cocos were cultivated in vitro, and subsequently, rapid amplification of polymorphic DNA was performed. To the best of our knowledge, this is the first study to examine the morphology of fruit bodies of W. cocos in Korea. W. cocos were cultured on PDA agar at different temperatures (12, 16, 20, 24, and 28oC) under 12-hour light (600 Lux) / 12-hour dark photoperiod condition for 1 month. Appearance of fruit body was the highest at 28°C condition in all the strains investigated. Honeycomb-like structure on sclerotia was observed in Andong 01, Andong 02, Andong 03, KFRI 1104, KFRI 1105, KFRI 1106, KFRI 1107, KFRI 1108, and ASI 13007 strains. The KFRI 1103 strain formed cosmos petal-like structure on sclerotia. The average size of basidiospores was recorded as 7.55 μm in height and 3.35 μ in width. This study was carried out to discriminate the geographical origin from Korea and Chinese Wolfiporia extensa. By proximate composition analysis, both were identified as similar, showed oxygen was 45.32-48.07%, carbon was 38.09-40.12%, hydrogen was 6.05~6.78% and nitrogen was 0.16-0.23%. Antioxidant activity was examined by DPPH free radical scavenging activity. No significant differences were found as well for the antioxidant activity between Korean and Chinese product. However, the contents of inorganic components ED-XRF (X-Ray Fluorescence Spectrometer) were significant different in Chinese and domestic W. extensa. The Potassium and iron in Chinese and domestic W. extensa were 47.60±8.78% and 14.5±3.86% as well as 33.14±17.27% and 9.13±4.83%, respectively. From the above resuls, the analysis of inorganic components by ED-XRF may be used for discrimination of the geographical origin of W. extensa.