전통 한약탕제인 녹용대보탕의 생리활성 효과

국문 초록 (Abstract)

여러 가지 생약재와 녹용이 첨가되어 보혈 강장제, 활력강화 및 성장촉진 효과가 있는 전통 한약탕제인 녹용대보탕에 대하여 in vitro에 의한 전자 공여작용과 지질과산화 억제효과 등의 항산화 활성과 아질산염 소거효과, tyrosinase 억제효과 그리고 혈소판 응집 억제활성 등을 분석하였다. 본 실험에서 사용한 한약탕제의 총 페놀함량은 151.3±2.6 ㎎%로 확인되었으며, 80.9%의 전자 공여효과를 나타냈다. 리놀레산을 이용한 지질과산화 억제효과는 88.1%로 나타났으며, pH에 따른 아질산염 소거효과를 비교 검토한 결과 시료의 아질산염 소거 효과는 산성 pH에서 70%정도의 활성을 나타냈으며 pH 1.2에서 가장 높고, pH 6.0에서 가장 낮은 소거효과를 나타내 pH가 높아질수록 그 활성이 낮았다. Tyrosinase 억제효과는 80%이상으로 나타났으며, SD rat의 혈소판을 ADP로 자극하였을 때 일어나는 혈소판 응집에 대한 저해활성을 측정한 결과 약 30%의 혈소판 응집 억제효과를 보였다. 또한 이들 억제효과는 농도 의존적으로 일어남을 알 수 있었다.

다국어 초록 (Multilingual Abstract)

This study was performed to examine in vitro biological activities such as antioxidative, nitrite scavenging effect, tyrosinase inhibitory effect and antithrombotic activity of a Korean traditional prescription, Nogyong-daebotang, composed of oriental medical herbs and antler, nourishing the blood, helthiness, strengthening of vital power and promotion of growth. The concentration of total phenolic compounds of the prescription sample was 151.3±2.6 ㎎%. The electron donating abilities (EDA) by reduction of 2.2'-diphenyl-1-picrylhydrazyl (DPPH) was 80.9%, inhibition rate of lipid peroxidation in thiocyanate method used linoleic acid was 88.1%. Nitrite scavenging effects of the sample were more than 70% at acidic pH, but it was pH dependent, showing the highest at pH 1.2 and the lowest at pH 6.0. Inhibition rate against tyrosinase of the sample was above 80%. Platelet aggregation induced by ADP (adenosine-5'-diphosphate) was inhibited up to 30% and the inhibitory effect was dependent on concentration of the sample.

목차 (Table of Contents)

• Abstract
  
• 서론
  
• 재료 및 방법
  
• 결과 및 고찰
  
• 요약
  
• 문헌

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