Bromobenzene으로 유도된 간 손상 마우스에 대한 Microcluster수의 효과 = Detoxification Effect of Microcluster-Water on Bromobenzene-Induced Liver Damaged Mice

국문 초록 (Abstract)

McW의 간 해독효과를 조사하였다. 실험군은 총 4개군 즉, 증류수급여군으로 대조군(DC)과 증류수를 3주간 섭취케 한 후 희생직전에 BB를 처리한 군(DB), McW 급여군으로는 McW 대조군(MC)과 McW를 3주간 섭취케 한 후 희생직전에 BB를 처리한 군(MB)의 4개 군으로 구분하였다. DC군과 MC군의 혈청 ALT 및 AST 활성은 뚜렷한 차이를 보이지 않았으나 MB군에서는 DB군에 비하여 유의하게 낮았다. 간 AH 활성은 모든 실험군에서 유의한 변동이 없었으나 GST 활성은 MC군이 DC군에 비하여, MB군이 DB군에 비하여 각각 높았다. 간 조직의 LPO 함량은 DC 및 MC군이 유사하였으며, DB 및 MB군 모두 DC군보다 유의하게 증가하였으나 그 증가율은 MB군이 DB군에 비하여 낮았다. McW의 전자공여능은 DW에 비하여 유의하게 높았다. 이상의 결과 McW는 간 해독효소의 일종인 GST의 활성을 증가시킴과 동시에 McW의 지속적인 섭취에 의한 항산화 작용에 의해 해독작용을 나타내는 것으로 생각되나 추후 계속적인 연구검토가 행해져야할 것이다.

다국어 초록 (Multilingual Abstract)

This study was conducted to investigate the hepatic detoxification effect of microcluster-water (McW). Animal experiments were divided into 4 groups: distilled water intake group (DC), distilled water intake-bromobenzene treated group (DB), McW intake group (MC), and McW intake-bromobenzene treated group (MB). There were no significant differences in alanine aminotransferase and aspartate aminotransferase activities between DC and MC groups, but the activities in MB group were significantly (p<0.05) lower than those in DB group. No apparent changes of aniline hydrolase activity were shown in all experimental groups, while glutathione S-transferase activity in MC and MB groups was higher than that in DC and DB, respectively. The content of hepatic lipid peroxide in DC group was similar to that of MC group. In addition, the contents in DB and MB groups were significantly (p<0.05) increased than that of DC group. The increasing rate in MB group was lower than that of DB group. Also, the electron donating activity of McW was significantly (p<0.05) higher than that of distilled water. From these results, it could be suggested that McW has the possibility of having detoxification effect of bromobenzene induced hepatic injury by increasing glutathione S-transferase, which is known as a kind of hepatiic detoxification enzyme.

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