Maternal Programming with a Cafeteria Diet Leads to Glucose Intolerance, Increased Insulinemia, Inflammation, and Intestinal Dysbiosis in Rat Offspring

다국어 초록 (Multilingual Abstract)

The intake of diets high in fat and sugar but low in protein, fiber, and vitamins during pregnancy can contributeto metabolic imbalance, immunological disorders, and intestinal barrier disruption in the offspring. This study investigatedthe effects of a cafeteria (CAF) diet, high in fat and sugar, consumed throughout pregnancy and lactation, onmetabolism, inflammation-related markers, and intestinal microbiota in rat offspring. Maternal programming was conductedfor more than 9 weeks in 16 female Wistar rats aged 8－10 weeks. They were divided into two diet groups: controland CAF, each with n=8. On the 21st day after birth, the offspring were weaned, and fecal samples were collected.
We conducted a series of glucose and insulin tolerance tests and analyzed serum metabolic markers [triglycerides (TGs),total cholesterol (TC), aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP),and gamma-glutamyl transferase (GGT)], inflammatory cytokines [monocyte chemoattractant protein-1 (MCP-1/CCL2)and interleukin 6 (IL-6)], and hormones (insulin, leptin, and active ghrelin). Gut microbial profiling was performed to assesscompositional changes. The CAF offspring exhibited higher levels of serum glucose, insulin, TGs, MCP-1, and IL-6compared with the control group. Moreover, the CAF diet altered the intestinal bacterial community structure, increasingthe abundance of Prevotellaceae and Lachnospiraceae while reducing Lactobacillaceae. Our results suggest that ingestinga CAF diet during maternal programming promotes adverse impacts on the offspring metabolism homeostasis, inflammatoryresponses, and intestinal microbiota composition.